Fig. 5From: Genome-wide transcriptome analysis reveals small RNA profiles involved in early stages of stolon-to-tuber transitions in potato under photoperiodic conditionsRelationship between miRNAs and their predicted targets by qRT-PCR at 4 d time-point under LD vs SD photoperiod conditions. Conserved miRNAs and their targets - (a) Stu-miR477a-5p and its target replication factor C, (b) Stu-miR319-3p and its target StTCP2, (c) Stu-miR479 and its target StGRAS. Novel miRNAs and their targets – (d) Stu-novel-miR302 and its target metal dependent phophohydrolase HD domain containing protein, and (e) Stu-novel-miR206 and its target exostosin family protein. f A correlation analysis shows the inverse relationship between miRNA expression and their putative target genes. U6 and EIF3e were used for normalization of miRNAs and target genes, respectively. The expression level of respective miRNA or target gene at LD4 time point was considered as 1 to measure relative expression at SD4 time point. Error bars represent standard error of means from two biological replicates. Asterisks (one, two and three) indicate significant differences at p < 0.05, p < 0.01, p < 0.001, respectively, using a Student’s t-test. ns = not significant at p < 0.05Back to article page