Fig. 6

Molecular identification and characterization of miR827 in Brassica napus. a Multiple alignment of mature miR827 sequences in monocot and dicot species. b The target genes of BnamiR827in the allotetraploid rapeseed genome. Mismatch nucleotides are indicated in red, and the G-U pair is denoted by green. The BnamiR827 and its target gene sequences are shown with lowercase and capital letters, respectively. c The secondary structure of the precursor sequence of BnamiR827. The mature sequence of BnamiR827 is boxed by dashed lines, and the vertical red line in the 5′ untranslated region of BnaNLA1s indicates the BnamiR827 target site. d Relative expression of BnamiR827 under short-term and long-term nitrogen (N) limitations. The rapeseed seedlings that were cultivated under high nitrate (NO₃⁻) (9.0 mM) for 10 d were then transferred to low NO₃⁻ (0.30 mM). At 0 h, 3 h and 72 h, the shoots and roots of the seedlings were individually sampled. Values denote means (n = 3), and error bars indicate standard error (SE) values