Fig. 6

Effect of CdDHN4-L and CdDHN4-S on lactate dehydrogenase (LDH) activity and alcohol dehydrogenase (ADH). a CdDHN4-L and CdDHN4-S at different concentrations was added to LDH. Relative enzyme activities are shown as percentage. Before-Activities of non-treated enzymes, After- the reaction solution was incubated 10 min at 50 °C. Values and bars represent means ± SD (three individual experiments). Different letters show significant differences (p < 0.05) as determined by ANOVA analysis among addition with BSA, CdDHN4-L and CdDHN4-S. b Cryoprotective activity was assayed with a ratio of 2:1 (test protein: LDH). LDH alone or in present of CdDHN4-L, CdDHN4-S or BSA protein were frozen in − 20 °C and then thawed at room temperature. c The heat-induced inactivation of ADH was carried out at 45 °C for 1 h, and enzyme activity was determined every 10 min. Activities of non-treated enzymes are standardized (100%)