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Table 1 Characteristics of meiotic prophase I and metaphase I stages in wheat and the efficiency of the MeioCapture protocol

From: MeioCapture: an efficient method for staging and isolation of meiocytes in the prophase I sub-stages of meiosis in wheat

Meiotic stage

Duration in CS (hour)a

Key events

No. of meiocytes isolated per hour, per person using the MeioCapture methodb

Condition of SACs

Pre-meiotic G2

–

DNA replication and chromosome organization

500 to 700

Intact

Leptotene

10.4

Chromosome association

1000 to 1200

Intact

Zygotene

3.4

Homologue pairing and SC formation

1000 to 1200

Intact

Pachytene

2.2

Crossing over and bivalent formation

1400 to 1500

Intact

Diplotene

0.6

Dissociation of SCs

1500

Intact

Diakinesis

0.4

Nuclear membrane disintegration and end of prophase I

300 to 400

Starting to disintegrate

Metaphase I

1.6

Chromosomes line up on metaphase plate

700 to 800

Partially disintegrated

  1. CS Chinese Spring, SC synaptonemal complex, SAC sporogenous archesporial column
  2. aThe duration of each meiotic stage provided is based on data from [9, 43, 44]
  3. b The total number of meiocytes isolated per hour varies depending on the mix of stages occurring in a spike