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Fig. 2 | BMC Plant Biology

Fig. 2

From: Analysis of a novel mutant allele of GSL8 reveals its key roles in cytokinesis and symplastic trafficking in Arabidopsis

Fig. 2

Callose deposition in the primary root of gsl8 mutants. Callose accumulation was visualized using aniline blue staining in five-day-old seedlings of WT, gsl8–1, gsl8–2 and essp8. a-d Bright blue lines and dots (white arrowheads) at the root tip represent callose deposition at the cell plate and plasmodesmata, respectively, in WT (a) and gsl8 mutants (b-d). e-h In the elongation zone of the primary root, callose deposition at plasmodesmata is detected as blue dots in the root hairs, cell wall and vascular tissue (white arrowhead) in the WT (e) and gsl8 mutants (f-h). i Quantification of callose levels in gsl8 mutants compared to WT Col-0. Values represent mean ± SEM (n = 10). **P < 0.01. Scale bars = 100 μm

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