Fig. 8
Gene expression profiles in transgenic Arabidopsis. a Heat map represent gene expression differences between MeGRXC15-OE and control (Vector) plants. The data was processed and normalized as described in Materials and Methods. Hierarchical clustering of significantly expressed genes is displayed by average linkage. The figure was drawn by TreeView software. b GO analysis of MeGRXC15-OE induced genes in Arabidopsis. Comparison of GO terms identified from the differentially expression genes identified in SAM analysis. GO tags were selected according to the significance (p-value < 10− 5). Numbers on y-axis indicate gene numbers of the GO tag. c Venn diagram showing the overlap between MeGRXC15-OE regulated genes in response to different stress and signals. d Venn diagram showing the MeGRXC15-OE induced transcription factors which involve in ABA and JA/ET signalling. Expression analysis of NCED (e), ABI1 (f), ABI2 (g), ABI5 (h), WRKY1 (i), WRKY46 (j), and WRKY53 (k) in transgenic Arabidopsis under drought and ABA treatments. Expression levels of target genes were normalized against vector control. Biological triplicates were averaged and significance of difference between treatments and control was analyzed using the Duncan’s multiple range tests. Different letters represent a significant difference at p < 0.05. Bars represent the mean ± standard error