Table 4 Primer sets used in this study
Primer set | Sequence 5′-3’ | Amplified target |
|---|---|---|
Ax | F: AGATGACTAAGCGGTTGGTTC | The genes of x-type HMW-GS on Glu-A1 locus |
R: CTGGCTGGCCAACAATGCGT | ||
Bx | F: ATGGCTAAGCGCCTGGTCCT | The genes of x-type HMW-GS on Glu-B1 locus |
R: TGCCTGGTCGACAATGCGTGC | ||
Dx | F: ATGGCTAAGCGGTTAGTCCT | The genes of x-type HMW-GS on Glu-D1 locus |
R: CTGGCTGGCCGACAATGCGT | ||
Y-type | F: ATGGCTAAGCGGTTGGTCCT | The genes of y-type HMW-GS |
R: GGCTAGCCGACAATGCGTCG | ||
Tublin | F: GGCTAGCCGACAATGCGTCG | β-tubulin gene of wheat |
R: GGCTAGCCGACAATGCGTCG |