Skip to main content
Fig. 3 | BMC Plant Biology

Fig. 3

From: Dual positive and negative control of Chlamydomonas PII signal transduction protein expression by nitrate/nitrite and NO via the components of nitric oxide cycle

Fig. 3

Effects of DEA-NONOate on the nitrate-induced expression of GLB1 gene and the levels of intracellular nitric oxide. a The effect of DEA-NONOate (10 μM, 50 μM or 100 μM) on the GLB1 transcripts accumulation was determined in 4 mM nitrate-induced C. reinhardtii cells of cw15–325 strain for 0.5 and 1 h. Nitrate-induced cells represent controls (set to 100%) without the added DEA-NONOate. Values are means ± SE of three biological replicates and three technical replicates and are given as expression level relative to a house-keeping gene RACK1 that has a value of 1. The increase in NIT1 transcript abundance was used as a positive control (insertion). b Fluorescence intensity due to intracellular NO was determined using 1 μM DAF-FM DA and was expressed as arbitrary units per μg protein. Cell autofluorescence was subtracted from the total fluorescence obtained. Insertion shows NO levels in the media (see Materials and Methods). Data are the means±SE from three independent experiments

Back to article page