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Fig. 2 | BMC Plant Biology

Fig. 2

From: Insights into the transcriptional and post-transcriptional regulation of the rice SUMOylation machinery and into the role of two rice SUMO proteases

Fig. 2

Analysis of the rice genes encoding the SUMO activating enzyme (SAE). The rice E1 is constituted by OsSAE1 encoding the regulatory subunit and OsSAE2 encoding the catalytic subunit. Maximum likelihood phylogeny for OsSAE1 (a) and OsSAE2 (b). Only nodes with bootstrap support > 75% show the correspondent bootstrap value. Os – Oryza sativa, Sc - Saccharomyces cerevisiae, At – Arabidopsis thaliana, Zm – Zea mays and Hv – Hordeum vulgare. c Venn diagram showing common CREs in the promoter region of both E1 subunit genes. Schematic representation of SAE alternative splicing forms showing three ASF for both OsSAE1 (d) and OsSAE2 (e). White boxes – untranslated regions (UTR); black boxes – exons; lines – introns. The proteins lengths are indicated. The difference in the sequence between OsSAE2.1 and OsSAE2.2/3 is indicated by an arrow. The arginine in OsSAE1 and the catalytic cysteines in OsSAE2 are depicted, as well as the domains and protein length for all ASFs. SAE2 proteins have four domains: adenylation domain where the catalytic cysteine domain is located, followed by the ubiquitin-like (UBL) and the C-terminal domains [108]. f Alignment of SAE2 proteins from different organisms Saccharomyces cerevisiae, Arabidopsis thaliana, and the two rice SAE2 ASF. The five amino acid insertion in OsSAE2.1 is highlighted. g Basal expression levels of the different ASF of OsSAE genes by qPCR in shoots (no pattern) and roots (patterned) in LC-93-4 (LC) and Nipponbare (Nipp). h and (i) Transcript level profile of ASFs of genes OsSAE1 and OsSAE2, respectively, in response to 15 μM ABA or 100 μM of GA

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