Fig. 4

Quantification of leaf damage on barley transformed (KD Icy2 1318 and 1399) and non-transformed (WT) plants, after 3 (light grey) and 7 (dark grey) days of M. oryzae infection (a). Damage was measured as mm² of injured foliar area and is represented as mean ± SE of seven old leaves measurements from seven independent plants per treatment. Effect of transgenic barley lines silencing the HvIcy2 gene (KD Icy2 1318 and 1399) and wild type (WT) plants on M. oryzae performance (b). Quantification of M. oryzae small subunit of ribosomal RNA (Mo28S-rRNA) mRNA expression levels, at 3 (light grey) and 7 (dark grey) days after M. oryzae infection. Total RNA was extracted after infestation and data were expressed as mRNA levels normalized to barley cyclophilin mRNA content. Different letters indicate significant differences (P < 0.01, one-way ANOVA Student Newman-Keuls test)