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Fig. 5 | BMC Plant Biology

Fig. 5

From: Differential response of silencing HvIcy2 barley plants against Magnaporthe oryzae infection and light deprivation

Fig. 5

Phenotypes of WT and HvIcy2 silencing (KD Icy2: 1318 and 1399 lines) barley plants grown in soil under continuous darkness or 16 h/8 h light/dark photoperiod for 14 days (a). Chlorophyll detection in the oldest leaf of transgenic and WT barley lines grown under darkness or 16 h/8 h photoperiod for 14 days (b). Leaf fragments from HvIcy2 silencing (KD Icy2: 1318 line) and WT plants were collected and observed under a Leica SP8 confocal microscope using the laser excitation lines 633 nm to detect the red autofluorescence from the chlorophyll (UV). The same images were taken under light field conditions (light) and the fluorescence signal overlap is documented (merged). Leaves were cut in two fragments, corresponding to apical and medium-basal section of the leaf, respectively. Scale bar, 200 μm

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