Fig. 5

Properties of pGbHyPRP1. a Histochemical analysis of GUS activity in Arabidopsis expressing the pGbHyPRP1-GUS chimeric gene. GUS activity was present in whole 7-day-old seedlings. b Location of cis-regulatory elements involved in hormone- and elicitor-responsive elements found in pGbHyPRP1. The conserved fungus-responsive elements ELI-box3 (− 216) were present in the promoter region of WRKY in the Populus, while the EIRE (elicitor responsive element) (−879) are the binding site of WRKY and required for elicitor responsiveness in the promoter of PR (pathogenesis-related protein) in the parsley [64, 65]. TC-rich repeat elements (− 502) are putatively involved in plant defense and stress response. Furthermore, ABRE (ABA-responsive element) (− 292), TCA-element and ERE (ethylene responsive element) (− 1128) have been reported in the upstream region of many genes that show regulated expression in response to ABA, SA and Et, respectively [26,27,28]. c Expression profiles of GbHyPRP1 in leaves of 15-day-old Pima90–53 seedlings subjected to SA, ABA, JA and ET. Control indicates water treatment. Three plants at each time point were sampled and analysed. Values are presented as the means ± SE in three independent experiments. Asterisks represent significant differences with respect to the control as determined by Tukey’s multiple comparisons test (*P < 0.05, ** P < 0.01; *** P < 0.001; ns = not significant)