Fig. 7

Analysis of lignin in stems of HyPRP1-silenced cotton plants after inoculation with V. dahliae. 10-day-old seedlings with two fully expanded cotyledons were used for VIGS. After two weeks later HyPRP1-silenced plants were inoculated with V. dahliae. Measurement and statistical analysis of the lignin content in cotton stems after inoculation with V. dahliae at 0 dpi (a), 7 dpi (b) and 14 dpi (c). At each time point of each treatment, three separate plants were examined. The results show means ± SE of values for three independent experiments. Asterisks indicate a statistically significant difference according to Sidak’s multiple comparisons test (**P < 0.01). Fluorescence microscopy of a 1-cm transverse stem section from control and HyPRP1-silenced cotton plants at 0 dpi (d&e), 7 dpi (f&g) and 14 dpi (h&i). Bar = 50 μm. Lignin autofluorescence was visualized following ultraviolet excitation at 365 nm