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Fig. 2 | BMC Plant Biology

Fig. 2

From: Cloning and characterization of norbelladine synthase catalyzing the first committed reaction in Amaryllidaceae alkaloid biosynthesis

Fig. 2

Phylogenetic relationships among several PR10/Bet v 1 proteins from a variety of plants. Phylogeny tree was based on the amino acid sequences for Betula verrucosa BvPR10, Betula platyphylla BpPR10, Papaver somniferum PsNCS, PsNCS2, Thalictrum flavum TfNCS, TfNCS2,TfNCS3, TfNCS4, TfNCS5, Chelidonium majus CmNCS1, Argenome mexicana AmNCS, Eschscholzia californica EcNCS1, EcNCS2, Coptis japonica CjNCS2 (formerly CjPR10A), Daucus carota DcPR10, Solanum tuberosum StPR10, Hyacinthus orientalis HoPR10, Pinus monticola PmPR10, Hordeum vulgare HvPR10, Oryza sativa OsPR10, Lily regale LrPR10, Sorghum bicolor SbPR10, Zea mays ZmPR10 and Hypericum perforatum Hyp-1. Accession numbers provided in Additional file 2. The evolutionary history was inferred using the Neighbor-Joining method [36] with bootstrap value 500. The optimal tree with the sum of branch length = 5.78745187 is shown. The tree is drawn to scale, with branch lengths in the same units as those of the evolutionary distances used to infer the phylogenetic tree. The evolutionary distances were computed using the p-distance method [43] and are in the units of the number of amino acid differences per site. The analysis involved 25 amino acid sequences. All positions containing gaps and missing data were eliminated. There were a total of 139 positions in the final dataset. Bootstrap values were added to the figure. Evolutionary analyses were conducted in MEGA 6 [38]

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