Skip to main content
Fig. 6 | BMC Plant Biology

Fig. 6

From: Cloning and characterization of norbelladine synthase catalyzing the first committed reaction in Amaryllidaceae alkaloid biosynthesis

Fig. 6

Extracted ion chromatograms showing the product of NpNBS enzyme assays. The tested substrates used were 3,4-dihydroxybenzaldehyde (300 μM) and tyramine (10 μM), the extracted ion chromatogram corresponds to standard NB or assays conducted with the non-induced protein fraction, the heat-denatured recombinant NpNBS enzyme; and the complete assay performed with recombinant NpNBS at pH 4 and pH 7. Parent ion mass-to-charge (m/z) of 260 for norbelladine and 258 for norcraugsodine were subjected to collision-induced dissociation analysis for identification and quantitation (Additional file 3)

Back to article page