Table 2 Primers used to generate pSPUTK constructs used for in vitro protein production
From: PISTILLATA paralogs in Tarenaya hassleriana have diverged in interaction specificity
pSPUTK cloning | ||
|---|---|---|
Gene | Fw | R |
AP3–1/2 | GATAGATCTATGACGAGGGGAAAGATTCAG | GATAGATCTTCATTCGAGCAAGTGGAAGG |
ThPI-1 | TTACCATGGGGAGAGGAAAGATAGAG | ATTATCGATCAGTCGATGACCAAAGACATGATC |
ThPI-2 | AATCCATGGGAAGAGGGAAGATAGAGATCAAAAG | TTTATCGATCAGACGATGTGTTGTAAATTGGGC |
Th2954 (AG) | ACGGCGTACCAAACGGAGTTG | TTACACTAACTGAAGTGGAGTGTG |
Th1528 (SEP3) | CATGCCATGGGAAGAGGTCGTGTTGAG | AAGATCGATCAATTGTTGTCATAAGGTAACCAAC |
Th18678 (SEP3) | CATGCCATGGGGAGAGGTCGAGTTG | AAGATCGATCAATTGTTGTCGTAAGGTAACCAAC |
Th21984 (SEP4) | ATGGGAAGAGGGAAAGTGGAGC | TCAGATCATCCAGCCGTGGAA |
Th2854 (SEP1/2) | ATGGGGAGGGGTAGGGTTG | TCAGAGCATCCAACCAGGG |
Th13754 (AP1) | ATGGGAAGGGGAAGGGTTCAG | TTATGTGAAGCAGCCAAGGTTGCAATC |