Fig. 4

Light micrographs showing transient expression of GFP constructs in aleurone layer. a) Graphical representation of constructs used for transient expression assays: i) Seed-specific expression glycinin promoter directing ER-localized GFP ii) Constitutive expression promoter 35S directing the expression of soluble GFP. b) GFP fluorescent micrograph of aleurone layer isolated from a previously produced stably transformed soybean plant expressing a seed-specific GFP-ER localized expression cassette. c) Soluble enhanced GFP driven by the constitutive promoter transiently expressed in aleurone tissue showing the spread of the GFP fluorescence between neighboring cells that is detectable in as little as 3 hours post-bombardment. d) ER-retained GFP driven by a seed promoter is expressed in aleurone cells and able to show the intracellular localization of the fluorescent signal. e) Aleurone tissue stained with the internal membrane stain DiOC6 to visualize the ER network for comparison to GFP-ER localized expression seen in stable transformation (b) and transient transformation (d)