Fig. 6

Relationships between total anthocyanin contents and transcript levels of the sixteen representative genes from Figs. 4 and 5. For each accession, the expression was determined in three developmental stages (S1 to S3) of fruit skin tissues. For the qRT-PCR assay, the mean was calculated from three biological replicates, each with three technical replicates (n=9). These replicates were then normalized relative to the expression of MdGADPH. The x-axis in each chart is the same and represents different Malus accessions, as indicated by names in the bottom panel, which are arranged in different clusters. The left y-axis represents relative expression levels determined by qRT-PCR