Skip to main content
Fig. 3 | BMC Plant Biology

Fig. 3

From: A cytosolic NAD+-dependent GPDH from maize (ZmGPDH1) is involved in conferring salt and osmotic stress tolerance

Fig. 3

Germination characteristics of the ZmGPDH1 transgenic lines in response to salt or osmotic stresses. a Reverse transcription PCR (RT-PCR) of ZmGPDH1 transcripts in the WT, atgpdhc2, ZmGPDH1 transformed atgpdhc2 (COM-1, COM-2) and transgenic WT (OE-1, OE-2) plants. ACTIN served as the internal reference. b The GPDH activities in ZmGPDH1 transgenic lines compared with the WT and atgpdhc2. c Morphological comparison among the WT, atgpdhc2, COM-1, COM-2, OE-1 and OE-2 lines grown in soil. Top panel, 28-day-old plants; bottom panel, 56-day-old plants. Bars = 200 mm. d The germination rate of the six lines under normal (control), NaCl or mannitol conditions at day 5 after imbibition. e Images of seeds germinated on either half-strength MS medium (control), medium with 100 mM NaCl or medium with 200 mM mannitol for 7 days. Bars = 50 mm. Asterisks indicated significant differences from the WT, as determined by Student′s t-test (*P < 0.05, ** P < 0.01)

Back to article page