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Fig. 5 | BMC Plant Biology

Fig. 5

From: The effects of mepiquat chloride on the lateral root initiation of cotton seedlings are associated with auxin and auxin-conjugate homeostasis

Fig. 5

Analysis of IAA amidohydrolase family of cotton. a Phylogenetic tree of amidohydrolase homologues from Arabidopsis (At), cotton (Gh). A Neighbor-Joining tree was generated by MEGA 6 software with a bootstrap value of n = 1000, based on full-length protein alignment by ClustalW. The numbers on the tree branches indicate bootstrap probability values. The scale bar represents the number of amino acid substitutions per site. b Relative expression of GhIAR3a, GhIAR3b and GhILR1 genes in various cotton tissues. c Expression of GhILR1, GhILL3a, GhILL6b, GhIAR3a and GhIAR3b (b) in control and MC-treated cotton seedlings. Values are the means ± SD (n = 4). Asterisks (*) and (**) indicate a significant difference (P < 0.05) and (P < 0.01), respectively, in comparison with the corresponding control

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