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Fig. 3 | BMC Plant Biology

Fig. 3

From: Overexpression of SrDXS1 and SrKAH enhances steviol glycosides content in transgenic Stevia plants

Fig. 3

Identification of transgenic Stevia plants overexpressing SrDXS1 (SrDXS1-OE) or SrKAH (SrKAH-OE). a Schematic maps of T-DNA region of pK7WG2D-SrDXS1 and pK7WG2D-SrKAH used for Stevia transformation. LB, left border; nptII, neomycin phosphotransferase marker gene under the terminator and promoter of nopaline synthase gene; T35S and P35S, terminator and promoter of the cauliflower mosaic virus gene respectively; attB2 and attB1, gene recombination sites; SrDXS1, Stevia 1-deoxy-d-xylulose-5-phosphate synthase 1; SrKAH, Stevia kaurenoic acid hydroxylase gene; EgfpER, enhanced green-fluorescent protein gene fused to endoplasmic reticulum targeting signal; ProlD, rol root loci D promoter; XbaI and HindIII, sites digested by XbaI and HindIII, respectively, for Southern blot analysis; Probe, probe used for Southern blot analysis. b Images of GFP signals from leaves and roots of representative SrDXS1-OE #6 or SrKAH-OE #4 under a fluorescence stereomicroscope. WT, wild-type. Scale bar = 1 mm. c Confocal images of the leaf underside and roots of WT, representative SrDXS1-OE #6 or SrKAH-OE #4. Auto, chlorophyll autofluorescence; GFP, GFP channel image; Light, light microscope image; Merged, merged image between Auto and GFP channels. Scale bar = 5 μm

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