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Fig. 4 | BMC Plant Biology

Fig. 4

From: Overexpression of SrDXS1 and SrKAH enhances steviol glycosides content in transgenic Stevia plants

Fig. 4

Genomic and expression analysis of transgenic Stevia plants overexpressing SrDXS1 (SrDXS1-OE) or SrKAH (SrKAH-OE). a and b SrDXS1 (a) or SrKAH (b) amplified from the gDNA of each transgenic Stevia lines. M1, 2-Log DNA ladder. PC, positive control amplified from the respective vector constructs. c and d Southern blot analysis of SrDXS1-OE (c) or SrKAH-OE lines (d). WT, wild-type. M2, DIG-labelled DNA molecular weight marker II. e and f Relative fold change in SrDXS1 (e) and SrKAH (f) transcript levels among the transgenic Stevia lines overexpressing SrDXS1 (SrDXS1-OE) and SrKAH (SrKAH-OE), respectively. Expression levels of both genes were normalized to that of actin and compared to that of wild-type (WT). The values are expressed as mean ± SE (n = 3). Student’s t-test was used for the analysis of statistical significance (*: p < 0.05, **: p < 0.01)

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