Fig. 7

Broccoli FLC1 promoter architecture. The sequences spanning the promoter region upstream of the start codon (− 1500 bp) were obtained by PCR using gene-specific primers. The PCR products were cloned into a TA-vector and 10 individual clones per genotype were sequenced. a Comparison of the FLC1 promoter sequences of Arabidopsis and broccoli genotypes. The distinct conserved motifs in the promoter regions are marked with vertical black lines. The gray boxes indicate identical nucleotides. b The EcoRI restriction enzyme site is denoted with triangles. c Validation of EcoRI recognition site by restriction enzyme digestion of PCR amplicons and gel electrophoresis analysis. d The AT-rich region is denoted with a black line. e The ANAERO1CONSENSUS element is denoted with a black line