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Fig. 2 | BMC Plant Biology

Fig. 2

From: The formation and accumulation of protein-networks by physical interactions in the rapid occlusion of laticifer cells in rubber tree undergoing successive mechanical wounding

Fig. 2

Immuno-histochemical localization of β-1,3-glucanase, chitinase and hevein in the bark at the tapping cuts soon after latex flow stopped. (A) The sections were simultaneously treated with polyclonal antibodies raised against β-1,3-glucanase, chitinase and hevein and triply labelled by the secondary RRX-conjugated goat anti-mouse (a), AMCA-conjugated goat anti-chicken (b), and FITC-conjugated goat anti-rabbit (c). (d) The merged image of (a), (b) and (c). (B) Magnification of the square areas in A. White arrows, protein-network; Gluc, β-1,3-glucanase; Chit, chitinase; Heve, hevein; PreH, the precursor of hevein, including pro-hevein and pre-prohevein

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