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Fig. 3 | BMC Plant Biology

Fig. 3

From: Transcriptional silencing of 35S driven-transgene is differentially determined depending on promoter methylation heterogeneity at specific cytosines in both plus- and minus-sense strands

Fig. 3

Cytosine methylation frequency in the 35S promoter of the S1 progenies. a-c Methylation frequency of plus-strand of the CaMV 35S promoter in S1:345, S1:208 ORN and the control 16c plants. N is the number of the clones used for the bisulfite sequencing. The x-axis is the position relative to the nucleotide distance from the transcription start sites. Bars under the x-axis in b and c are the nucleotide position of the CaMV 35S promoter sequence integrated in the CMV-A1 vectors. d Summary of the results (a to c) to show differences in CHH, CHG, CG and total methylation. The asterisks indicate a statistical significance in methylation frequencies between S1:345 and S1:208 ORN by two-tailed Fisher’s exact test (** P < 0.01)

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