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Fig. 5 | BMC Plant Biology

Fig. 5

From: Transcriptional silencing of 35S driven-transgene is differentially determined depending on promoter methylation heterogeneity at specific cytosines in both plus- and minus-sense strands

Fig. 5

Cytosine methylation frequency in the 35S promoter in the Vein tissues of S2:208 RED and S2:208 ORN. a Comparison of methylation status in the plus-sense of 35S promoter between S2:208 RED and S2:208 ORN. b Summary of the results of a to show differences in CHH, CHG, CG and total methylation. c Comparison of the methylation status in the minus-sense sequence of the 35S promoter between S2:208 RED and ORN. Asterisks indicate cytosine residues that are significantly different in methylation frequency between RED and ORN as explained in Fig. 6b. d Summary of the results of c to show differences in CHH, CHG, CG and total methylation. N is the number of the clones used for the bisulfite sequencing. The x-axis shows the position relative to the transcription start site (+ 1). The asterisks in (b) and (d) indicate a statistical significance in methylation frequencies by two-tailed Fisher’s exact test (* P < 0.05, ** P < 0.01)

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