Gene | Purpose | Annealing temperature. (°C) | PCR product length (bp) DNA/cDNA | Forward primer (5′ → 3′) | Reverse primer (5′ → 3′) |
---|---|---|---|---|---|
TaMyc-A1 | qRT-PCR, sequencing | 60 | 283/177 | AACCATGTCATTTCGGAGAGGA | GGCCGCCCTGTTGGATC |
PCR, sequencing | 60 | −/1707 | ATGGCGCTGCCAGTAGTTCGTC | TCATGGCCTGCGAATAGCTCTCT | |
cloning | 50 | 292/− | TTATTAGATTAAGTATAGTTTTTTTGGTAGAA | CAACATCAAAATACTATACTACACTAACA | |
cloning | 53 | 231/− | TGTTAGTGTAGTATAGTATTTTGATGTTG | ACCATAACCCATAAATAAAAAAAAAACTTCC | |
cloning | 57 | 309/− | GGAYGATAGGTTGGTTTTTGAGTTTTTTG | CCATTACCACACTATTTCCTTCCTTCA | |
TaMyc-A2 | qRT-PCR, sequencing | 60 | 334/220 | AACCATGTCATTTCGGAGAGGA | TCTTCCCGCCGACTTCATGA |
PCR, sequencing | 60 | −/1697 | ATGGCGCTGCCAGTAATTCGTC | TCAGCGCCTGCGTATAGCTTTCT | |
TaMyc-B1 | qRT-PCR, sequencing | 60 | 347/198 | AACCATGTCATTTCGGAGAGGA | GAGTTTTCGGACGGCTGTTG |
PCR, sequencing | 60 | −/1692 | ATGGCGCTGTCAGTAGTTCGTC | TCAGCGCCTGCGTATGGCTCT | |
TaMyc-D1 | PCR, sequencing | 60 | 504/244 | GCTCATCGTGCTTTGCGG | GCCGCCCTGTTGGATTCT |
TaMyc-D2 | qRT-PCR, sequencing | 60 | 341/188 | AACCATGTCATTTCGGAGAGGA | ACGGCTGTTCCGGCCA |
PCR, sequencing | 63 | −/1665 | ATGGCGCTGCCGGTAGTTCATC | TCATCCGGGTTCCACGGCTCCA |