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Table 2 Gene-specific primers used for amplification of the TaMyc genes in current study. Primer pairs used for promoter region amplification of TaMyc-A1 are underlined

From: Myc-like transcriptional factors in wheat: structural and functional organization of the subfamily I members

Gene

Purpose

Annealing temperature. (°C)

PCR product length (bp) DNA/cDNA

Forward primer (5′ → 3′)

Reverse primer (5′ → 3′)

TaMyc-A1

qRT-PCR, sequencing

60

283/177

AACCATGTCATTTCGGAGAGGA

GGCCGCCCTGTTGGATC

 

PCR, sequencing

60

−/1707

ATGGCGCTGCCAGTAGTTCGTC

TCATGGCCTGCGAATAGCTCTCT

 

cloning

50

292/−

TTATTAGATTAAGTATAGTTTTTTTGGTAGAA

CAACATCAAAATACTATACTACACTAACA

 

cloning

53

231/−

TGTTAGTGTAGTATAGTATTTTGATGTTG

ACCATAACCCATAAATAAAAAAAAAACTTCC

 

cloning

57

309/−

GGAYGATAGGTTGGTTTTTGAGTTTTTTG

CCATTACCACACTATTTCCTTCCTTCA

TaMyc-A2

qRT-PCR, sequencing

60

334/220

AACCATGTCATTTCGGAGAGGA

TCTTCCCGCCGACTTCATGA

 

PCR, sequencing

60

−/1697

ATGGCGCTGCCAGTAATTCGTC

TCAGCGCCTGCGTATAGCTTTCT

TaMyc-B1

qRT-PCR, sequencing

60

347/198

AACCATGTCATTTCGGAGAGGA

GAGTTTTCGGACGGCTGTTG

 

PCR, sequencing

60

−/1692

ATGGCGCTGTCAGTAGTTCGTC

TCAGCGCCTGCGTATGGCTCT

TaMyc-D1

PCR, sequencing

60

504/244

GCTCATCGTGCTTTGCGG

GCCGCCCTGTTGGATTCT

TaMyc-D2

qRT-PCR, sequencing

60

341/188

AACCATGTCATTTCGGAGAGGA

ACGGCTGTTCCGGCCA

 

PCR, sequencing

63

−/1665

ATGGCGCTGCCGGTAGTTCATC

TCATCCGGGTTCCACGGCTCCA