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Fig. 2 | BMC Plant Biology

Fig. 2

From: A repeat region from the Brassica juncea HMA4 gene BjHMA4R is specifically involved in Cd2+ binding in the cytosol under low heavy metal concentrations

Fig. 2

Expression profiles of BjHMA4 in B. juncea. a Expression analyses of BjHMA4 genes under normal conditions. The total RNA was extracted from the roots, stems, and leaves of three-week-old B. juncea plants. b The expression levels of BjHMA4 in the roots under different ZnCl2 treatment periods. c, d Expression analyses of BjHMA4 genes in the roots and shoots in response to heavy metal treatments. Three-week-old WT B. juncea plants were exposed to 400 μM NiCl2, 200 μM CdCl2, or 2000 μM ZnCl2 for 6 h. e, f Expression analyses of BjHMA4 genes in the roots and shoots under Mn-, Cu- or Zn-deficient conditions. Two-week-old B. juncea plants were transferred to Mn-, Cu- or Zn-depleted media for one week. The data represent the means of three biological replicates. Different letters above the columns indicate significant differences (P < 0.05) between seedling groups under the same stress treatment

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