Fig. 1

Multicolor FISH identification using a probe mix of C. clementina somatic metaphase chromosomes that were successively hybridized onto the same metaphase plate. The chromosomes were counterstained with DAPI (blue), and probe signals were pseudocolored in different colors (red, green and yellow). For a better visualization, the FISH signals in (b–d), (f–h), and (j–k) were digitally separated from the merged graphs (a), (e) and (i), respectively. (b) CL1; (c) CL17; (d) CL3; (f) CL2; (g) 5 s rDNA; (h) CL4; (j) 45 s rDNA; (k) TTTAGGG; (l) image showing the morphology of metaphase chromosomes; (m) the chromosomes in the karyogram extracted from the respective photomicrographs (a), (e), and (i), were numbered and ordered on the basis of their total descending length, FISH signals and morphological characteristics. Individual chromosome pairs of C. clementina were discriminated after three rounds of multicolor FISH