Fig. 3

Sequence alignment and phylogenetic analysis of PbMYB12. a Phylogenetic analysis of typical flavonoid-regulating MYBs from different species. These MYBs were classified into three major groups. Pear PbMYB12a and PbMYB12b are marked by asterisks. The MYB protein sequences in other species were obtained from the NCBI database. Their protein accessions in the NCBI database are as follows: Arabidopsis PRODUCTION OF ANTHOCYANIN PIGMENT 1 (AEE33419.1), Arabidopsis PRODUCTION OF ANTHOCYANIN PIGMENT 2 (AAG42002.1), pear PbMYB10 (ALU57825.1), pear PbMYB10b (ALU57826.1), apple MdMYB12 (ADL36755.1), apple MdMYB9 (ABB84757.1), pear PbMYB9 (ALU57827.1), Arabidopsis TT2 (OAO91653.1), grapevine VvMYBF1 (ACV81697.1), apple MdMYB22 (AAZ20438.1), Mimulus LAR1 (ALP48586.1), Arabidopsis PRODUCTION OF FLAVONOL GLYCOSIDES 1 (PFG1) (OAP07166.1), Arabidopsis PFG2 (OAP04683.1) and Arabidopsis PFG3 (OAO93308.1). The phylogenetic tree was constructed by MEGA7.0 using a bootstrap test of phylogeny with the Neighbor-joining test and default parameters. b Protein sequence alignments of PFG-type MYBs from different species. The conserved R2 and R3 domains are underlined. Previously described SG7–1 and SG7–2 motifs are boxed. Identical and similar amino acids are shaded. The multiple alignment was performed using ClustalW