Fig. 6

PbMYB12b-regulated flavonol biosynthesis in pear fruit. a Flavonol glycoside contents in ‘Zaosu’ fruit transiently overexpressing PbMYB12b. b The expression patterns of PbMYB12b and flavonol biosynthetic genes in ‘Zaosu’ fruit transiently overexpressing PbMYB12b. c Flavonol glycoside contents of ‘Red Zaosu ‘fruit transiently silencing PbMYB12b using VIGS. d The expression patterns of PbMYB12b and flavonol biosynthetic genes in ‘Red Zaosu’ fruit transiently silencing PbMYB12b using VIGS. The pear fruit in DP were infiltrated and sampled at 48 h after infiltration. The control fruit were infiltrated by pCambia1301-GUS (OE-GUS) and original TRV2, respectively. Data are the means ± SDs of three biological replicates. Asterisks indicate significant differences as assessed by Student’s t test: ^*P < 0.05, ^**P < 0.01