Fig. 3

The GhWRKY27 gene accelerates age-triggered leaf senescence in transgenic Arabidopsis. a qRT-PCR analysis of GhWRKY27 transcript levels in WT and three transgenic lines. b Senescence phenotypes of WT plants and three transgenic lines. c Chlorophyll content of WT plants and three transgenic lines. d-g qRT-PCR analysis of the transcript levels of the SAGs AtNAP, AtORE1, AtSAG12 and AtSAG13 in Arabidopsis. AtUBQ10 was employed as a reference gene. Values represent the means ± SDs. The significance of the data was determined using Student’s t-test (^**P < 0.01). The experiment was conducted with three repetitions