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Fig. 1 | BMC Plant Biology

Fig. 1

From: Endoplasmic reticulum–localized UBC34 interaction with lignin repressors MYB221 and MYB156 regulates the transactivity of the transcription factors in Populus tomentosa

Fig. 1

The specific interaction between PtoMYB221 and the N35 protein in a split-ubiquitin Y2H system. Bait protein PtoMYB221 fused to Cub was co-expressed in yeast NMY51 with prey proteins, NubI, NubG, or the NubG:N35 protein. NubI is a positive prey control with strong affinity between wild-type NubI and Cub, whereas NubG is a negative prey control with weak affinity between mutant NubG and Cub. Interaction of the bait was shown with positive control NubI and the test NubG:N35 fusion protein but not with the negative control NubG, as indicated by growth of the transformed strain on synthetic defined medium without Trp, Leu, and His and by the activity of β-Gal

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