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Fig. 8 | BMC Plant Biology

Fig. 8

From: Endoplasmic reticulum–localized UBC34 interaction with lignin repressors MYB221 and MYB156 regulates the transactivity of the transcription factors in Populus tomentosa

Fig. 8

PtoMYB221 targets to the AC cis-elements of monolignol pathway genes and its repression activity is reduced with the involvement of PtoUBC34. a. Schematic representation of the effector and reporter plasmids used for evaluation of the affinity of PtoMYB221 to AC cis-elements in a dual LUC assay. b. Synthetic AC elements tested in dual LUC assay. c. LUC activity assay driven by PtoMYB221 binding to the AC elements in poplar mesophyll protoplasts. AC-I, AC-II, AC-III, and AC-IV are the reporters containing three copies of the ACx elements fused to the CaMV35S promoter and LUC reporter gene. d. PtoUBC34 reduced the repression activity of PtoMYB221. 2.4 μg of the effector plasmids PtoMYB221, 600 ng of PtoUBC34 or PtoUBC34m, along with the reporter plasmid and internal control plasmid, were used for co-transfection. Three independent experiments show similar results. Error bar represents standard deviation of three independent batches of P. tomentosa mesophyll protoplast transfections from one of the experiments. Significance was tested with one-way ANOVA to evaluate the roles of the effectors on the LUC expression. The different alphabets above the bar indicates statistically significant differences with p value < 0.05, while the same alphabet mean no significant differences

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