Skip to main content
Fig. 5 | BMC Plant Biology

Fig. 5

From: Temporal and spatial detection of Candidatus Liberibacter asiaticus putative effector transcripts during interaction with Huanglongbing-susceptible, −tolerant, and -resistant citrus hosts

Fig. 5

Comparison of effector expression in the leaf and root of citron (a), Duncan (b) and Cleopatra (c) infected by Ca. L. asiaticus. Photographs display the health of Ca. L. asiaticus-infected plants (a-c) over a year after inoculation (right), in comparison to their uninfected controls (left). The Ca. L. asiaticus was quantified by qPCR amplifying 16 s rDNA from 100 ng of DNA template. A standard curve method with the function Log (copy number) = − 0.289*Ct + 11.66 was used to calculate 16 s copy numbers (d). The RNA was isolated from the same group of samples for effector analysis by RT-qPCR, from which the number of effectors detected was compared between leaf and root tissues in citron, Duncan and Cleopatra (E). Analysis was based on three biological replicates and was analyzed by Student’s t-test (p < 0.05) and significant difference between leaf/root pairs is indicated by an asterisk

Back to article page