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Fig. 6 | BMC Plant Biology

Fig. 6

From: VvmiR160s/VvARFs interaction and their spatio-temporal expression/cleavage products during GA-induced grape parthenocarpy

Fig. 6

Functional analyses of the VvMIR160c and VvARF10 promoters. a Schematic diagram of the pBI121-pVvmiR160c/VvARF10-GUS constructs. pBI121-p1VvMIR160c-GUS and pBI121-p1VvARF10-GUS constructs. The 1.5 kb promoter regions of VvMIR160c and VvARF10 were cloned into pBI121 to replace the 35SCaMV promoter and were used to drive the GUS gene. pBI121-p2VvMIR160c-GUS constructs. The 1442 bp promoter fragment region of VvMIR160c without including the GA response element (59–1500 bp) was cloned into pBI121 to replace the 35SCaMV promoter and was used to drive the GUS gene. pBI121-p2VvARF10-GUS constructs. The 937 bp promoter fragment region of VvmiR160c without including the GA response element (564–1500 bp) was cloned into pBI121 to replace the 35SCaMV promoter and was used to drive the GUS gene. b and c Histochemical staining pattern (b) and GUS activity (c) of tobacco leaves in response to different treatments. The fully expanded leaves from 6-week-old tissue culture plants of tobacco were infiltrated with Agrobacterium carrying different constructs. The infiltrated seedlings were then moved back to the environmental chamber and kept in the dark for 3 d. Treatment on tobacco leaves with 30 μM GA and 50 μM GA were performed 2 days later. The uniformly sized leaves were used in infiltration experiment. The presented images of leaves in (b) are representative of the results obtained from three independent experiments. Data shown in (c) represent the mean ± SD of three independent experiments (n = 3). Different letters denote significant differences between the constructs and the treatments (P < 0.05), positive control (pBI121); negative control (pBI101)

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