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Fig. 8 | BMC Plant Biology

Fig. 8

From: Identification and functional characterization of three new terpene synthase genes involved in chemical defense and abiotic stresses in Santalum album

Fig. 8

In vitro enzymatic assays of recombinant SaTPS3. In vitro enzyme assays of recombinant SaTPS3 using FPP (a) or GPP (b) as the substrate in the presence of Mg2+. The reaction products were analysed by GC-MS. The peaks marked with numbers were identified by comparison of their mass spectra with those in library data and a comparison of their retention index. Peaks in a: 1, cedrene, 2, unknown, 3, (E)-β-farnesene, 4, unknown, 5, γ-bisabolene, 6, (E)-nerolidol, 7, (E,E)-farnesol. Peaks in b: 1, myrcene, 2, limonene, 3, unknown, 4, β-ocimene, 5, linalool, 6, α-terpineol, 7, geraniol. Mass spectra for the major products and corresponding authentic standards are shown, respectively. m/z, mass-to-charge ratio

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