Fig. 8From: Identification and functional characterization of three new terpene synthase genes involved in chemical defense and abiotic stresses in Santalum albumIn vitro enzymatic assays of recombinant SaTPS3. In vitro enzyme assays of recombinant SaTPS3 using FPP (a) or GPP (b) as the substrate in the presence of Mg2+. The reaction products were analysed by GC-MS. The peaks marked with numbers were identified by comparison of their mass spectra with those in library data and a comparison of their retention index. Peaks in a: 1, cedrene, 2, unknown, 3, (E)-β-farnesene, 4, unknown, 5, γ-bisabolene, 6, (E)-nerolidol, 7, (E,E)-farnesol. Peaks in b: 1, myrcene, 2, limonene, 3, unknown, 4, β-ocimene, 5, linalool, 6, α-terpineol, 7, geraniol. Mass spectra for the major products and corresponding authentic standards are shown, respectively. m/z, mass-to-charge ratioBack to article page