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Fig. 4 | BMC Plant Biology

Fig. 4

From: Nitric oxide synthase-mediated early nitric oxide burst alleviates water stress-induced oxidative damage in ammonium-supplied rice roots

Fig. 4

ROS and ONOO− accumulation in root apices of rice seedlings treated with NO donor (SNP) and either receiving non-water stress (Con) or subjected to water stress using PEG. After 3 h, O2.- (a), H2O2 (b), and OH− (c) levels in rice seedlings roots were measured by spectrophotometry. ONOO- production expressed as relative fluorescence (d). The accumulation of ONOO- was detected with 10 μΜ aminophenyl fluorescein (e), Bar =3 00 μm. Fluorescence images and relative fluorescence intensity were analyzed as described in Fig. 2 for NO determination. Values represent means ± standard error (SE) (n = 6). Different letters indicate significant differences at P < 0.05. Con indicates control treatment for each N nutrition, i.e., plants receiving non-water stress. FW: fresh weight

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