Gene | Description | ESTs Saccharum spp. | Locus Sorghum bicolor | Primer forward (5′-3′) | Primer reverse (5′-3′) | Amplicon (bp) | Efficiency (%) |
---|---|---|---|---|---|---|---|
NADP-ME | NADP-malic enzyme | comp88554_c0_seq11 | Sb03g003230 | GTGAGGCCTGCCAGAAGTAT | CTAGGACCTTCCCCTTGTCC | 84 | 99 |
NAD-ME1 | NAD-malic enzyme | comp79553_c1_seq21 | Sb01g017790 | TACAGGGGACAGCTGGAGTT | CTCCCACAACGACGATCTTT | 102 | 95.5 |
NAD-ME2 | NAD-malic enzyme | comp83394_c0_seq41 | Sb02g033920 | CAGCAGTTCCCTGAACATCA | CGCTGGCCTAATGTTATCGT | 118 | 92.5 |
PEPCK | Phosphoenol-pyruvate carboxykinase | comp81929_c0_seq51 | Sb01g040720 | CTGTCGCAGGAGAAAGAACC | CAGATTTGTCGGCGTAGTCA | 112 | 100 |
AspAT1 | Aspartate aminotransferase | comp82581_c0_seq11 | Sb03g035220 | GCACAGTCCTCATGCTCAAA | AATAGCGAGCAAGTGGCATT | 97 | 93 |
AspAT2 | Aspartate aminotransferase | comp83051_c1_seq51 | Sb04g036060 | CGCGTTTAACAAAGCAACAG | CCGAGAGAGACTGAATTGTAGC | 94 | 97 |
AlaAT | Alanine aminotransferase | TC1135192 | Sb01g023750 | TGCCACAGAAAGCAATTGAG | GGACCACGACAATTCCAGTT | 99 | 98.5 |
GAPDH | Glyceraldehyde-3-phosphate dehydrogenase | SCBFFL4116A05.g3 | – | CACGGCCACTGGAAGCATCCTCAG | TCCTCAGGGTTCCTGATGCC | 101 | 97.5 |