Fig. 4

Immunohistochemistry of the grafted hypocotyl sections, first time frame – homogalacturonan (LM20 and LM19 epitopes), extensins (JIM11 and JIM20 epitopes), and AGPs (JIM13 epitope). a – scion, epitope present in the walls of the dividing cells (arrows), no epitope detected on the surface of the callus cell protruding outside the scion (arrowhead). a′ a, Calcofluor White. b – scion, epitope present in the walls of the dividing cells (full arrows), in the cell compartments (arrow), and on the surface of the callus cells protruding outside the scion (arrowhead). c – stock, epitope detected in the walls (arrow) and on the surface of the cells (arrowheads). d – scion, epitope present in the cellular compartments of some cortical and endodermal cells, near the cut (arrows). e – scion, epitope present in the endodermal cells (full arrow) and cells next to the vessels (arrows), no epitope detected in the callus cells protruding outside the scion (arrowhead). c Calcofluor White en endodermis. Scale bars: a–c = 10 μm; d and e = 50 μm