Fig. 2
From: RcPAL, a key gene in lignin biosynthesis in Ricinus communis L.
Amplification profiles of RcPAL CDs, digested vectors and transformed EHA105. a Amplification of RcPAL CDs. Lane M: Marker DL2000; lanes 1~2: overexpression CDs; lanes 3~4: antisense CDs. b Amplification of pYLRNAi-PAL+. Lane M: Marker DL2000; lane 1: negative control pYLRNAi.02; lane 2: positive control pMD-PAL; lanes 3~10: recombinant vectors, among which 4, 5, 7, 9, 10 were positive. c Double digestion of pYLRNAi-PAL+. Lane M: Marker DL2000; lane 1: double digestion products. d Amplification of Agrobacterium EHA105 with pYLRNAi-PAL+. Lane M: Marker DL2000; Lane 1: positive control pYLRNAi-PAL+; lane 2~7: transformed Agrobacterium EHA105, among which 3, 5, 6 and 7 were positive. e Amplification of pYLRNAi-PAL−. Lane M: Marker DL2000; lane 1: negative control pYLRNAi.02; lane 2: positive control pMD-PAL; lanes 3~10: recombinant vectors, among which 4, 5, 8, 9, 10 were positive. f Double digestion of pYLRNAi-PAL−. Lane M: Marker DL2000; lane 1: double digestion products. g Amplification of Agrobacterium EHA105 with pYLRNAi-PAL−. Lane M: Marker DL2000; lane 1: positive control pYLRNAi-PAL−; lane 2~7: transformed Agrobacterium EHA105, all were positive