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Fig. 8 | BMC Plant Biology

Fig. 8

From: Genome constitution and evolution of Elytrigia lolioides inferred from Acc1, EF-G, ITS, TrnL-F sequences and GISH

Fig. 8

Results of FISH and GISH in Elytrigia lolioides. a and g: PI 440059, b and h: W6–26567, c-f and i: W6–26586. a-c: Used St2–80 as probe, 28 chromosomes were labeled as St type (arrows). The rest of the chromosomes were labeled as non-St type in three accessions of E. lolioides, respectively. d: Total genomic DNA of Pseudoroegneria libanotica was labeled with fluorescein-12-dUTP (green) as probe, 28 chromosomes were hybridized with P. libanotica probe (St genome). e: Total genomic DNA of P. libanotica labeled with fluorescein-12-dUTP (green) and total genomic DNA of Lophopyrum bessarabicum labeled with Texas-red-5-dCTP (red) as probes, 28 chromosomes were hybridized with the P. libanotica probe (St genome) and no any E genome signals were displayed on the chromosomes. f: Total genomic DNA of P. libanotica labeled with fluorescein-12-dUTP (green) and total genomic DNA of Hordeum bogdanii labeled with Texas-red-5-dCTP (red) as probes, 28 chromosomes were hybridized with the P. libanotica probe (St genome) and the rest were hybridized with the H. bogdanii probe (H genome). g-i: Total genomic DNA of H. bogdanii labeled with fluorescein-12-dUTP (green), 14 chromosomes were hybridized with the H. bogdanii probe (H genome) in three accessions of E. lolioides, respectively. However, in accession of W6–26586, one chromosome which belongs to H genome did not hybridized H genome signals on the pericentromeric regions (arrow). Insets showed the magnified view of the signal on this chromosome

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