Table 2 Primers used for RT-qPCR
Gene | Full name | Forward primer (5′-3′) | Reverse primer (5′-3′) |
|---|---|---|---|
SlGPI | Glucose-6-phosphate isomerase | AGAGGGTTCGCAGTGGTTCCTGGGTT | CCTACCCAGTCCCAGAAAGCGAATGC |
SlPMI | Phosphomannose isomerase | TGGACATGCTAATGGCGGAGGA | AACGAGTGGCTGCTCCTTATGC |
SlPMM | Phosphomannomutase | TGTTGGAGTTGTTGGAGGTTCTGAC | CTCCCAATAGGCGACACATTTAGCA |
SlGMP | GDP-D-mannose pyrophosphorylase | AAACCTGAAATCGTGATGTGAGA | TGAAGAAGAGGAGAACTGGAAAC |
SlGME1 | GDP-mannose 3′,5′-epimerase 1 | TCCGACTTCCGTGAGCCAGTAA | CGTGTTGTCTGAGTTGCGACCA |
SlGGP | GDP-L-galactose-1-phosphate phosphorylase 1 | AGGCTTCTCGCAGAGGTCTCAC | CCATCAGTGCCGTCCTCGTTCT |
SlGP1 | L-Galactose-1-phosphate phosphatase 1 | GTGGCATGTGGAAGGCTTGAACT | CGGCTACACGTTGTGATGTGATGT |
SlGaLDH | L-Galactose dehydrogenase | ATGAGACACTTCCCGCCCTTCTAA | CTTCGTCCATTCCAGTCGTTGCTA |
SlGLDH | L-Galactono-1,4-lactone dehydrogenase | TGCTCCGTTCCTTCGCTTCCA | TGGCGGCAGCAGAACCTAAGA |
SlMIOX | Myo-Inositol oxygenase | GGGCTTCCTCAATGGGCTGTT | TGTCCTCCTCATTCATCAAGTGTGT |
SlMDHAR | Monodehydroascorbate reductase | GCTGGTGGTGAGAGGCAAACA | GGCAAGAGAAGGAGTGAACAGTCT |
SlDHAR1 | Dehydroascorbate reductase 1 | AGCAGGCTCTCCTTGATGAACTAA | TCAGGCACACTCCACTTCTTGAA |
SlAO | L-ascorbate oxidase | CAGGATGGCTCAGAGTGTTGCTAT | GGCGATCAGGTAAGGCGTATGG |
SlAPX | Ascorbate peroxidase | TGGGAGGGTGGTGACATATTTT | TTGAAGTGCATAACTTCCCATCTTT |
SlPR1 | Pathogenesis-related protein | GTGTCCGAGAGGCCAGACTA | ATTGTTGCAACGAGCCCGA |
SlPR2 | Beta-1,3-glucanase | TCCAGGTAGAGACAGTGGTAAA | CCTAAATATGTCGCGGTTGAGA |
SlPR5 | Thaumatin-like protein | GGCCCATGTGGTCCTACAAA | GGCAACATAGTTTAGCAGACCG |
SlSOD | Superoxide dismutase | CTTCACCACAACCAGCACTACCAA | TCCAGGAGCAAGTCCAGTTATACGA |
SlPOD | Peroxidase | TTGGAGTGTCTCGTTGCTCA | TTCACCAGCACTCCCTGTCT |
SlCAT2 | Catalase | CAAGTTCGCCATGCTGAGGTGTAT | AGCCTGAGACCAGTATGTGATCCAA |
Tubulin | Tubulin | TGACGAAGTCAGGACAGGAA | CTGCATCTTCTTTGCCACTG |
TYLCV | TYLCV DNA detected | ATGTCGAAGCGACCAGGCGATATAAT | TTAATTTGATATTGAATCATAGAAAT |