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Fig. 4 | BMC Plant Biology

Fig. 4

From: Development and characterisation of interspecific hybrid lines with genome-wide introgressions from Triticum timopheevii in a hexaploid wheat background

Fig. 4

FISH validation of the SNP genotyping of wheat-T. timopheevii introgression lines. Oligos pSc119.2–1 (green) and the Afa family (red) were used as FISH probes. a known FISH karyotype of wheat [56] (b) FISH karyotype of T. timopheevii accession P95–99.1-1 (based on that published by [37]) (c) FISH karyotype of line BC3F2–114-1 showing a homozygous introgression from T. timopheevii linkage group 5G into wheat chromosome 5B (d) FISH signals in the metaphase spread of line BC3F2–114-1 used to make the karyotype with white arrows indicating chromosomes T5GS.5GL-5BL (e) GGT bar diagram of line BC3F2–114-1 showing the T. timopheevii introgression from linkage group 5G in red markers while the wheat alleles are represented in blue

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