Fig. 4

Differences of shikimate metabolism among extracted tetraploid wheat (ETW), its common wheat donor (line TAA10, genome BBAADD), Ae. tauschii (line TQ18), an extant tetraploid wheat line (line ALTAR81, genome BBAA), and a resynthesized allohexaploid wheat (XX329, genome BBAADD) obtained by crossing ETW (maternal parent) and TQ18 (paternal parent). a shikimate content. b Changes in metabolic network of shikimate in ETW. c Shikimate metabolism pathway. d Gene expression of shikimate metabolism pathway. The expressional values are means of four biological replicates. Asterisks indicate significant differences (t-test, P < 0.05) between ETW and each wheat line. DAHP1: 3-deoxy-7-phosphoheptulonate synthase 1, DHQ: 3-de-hydroquinic acid synthase, DHQD: 3-dehydroquinic acid dehydratase, SK: Shikimate kinase, EPSPS: 5-enolpyruvylshikimate-3-phosphate synthase, CS: Chorismate synthase