Fig. 5

Pollen phenotyping in iFRK3 mutants. a. The pollen grains were examined by acetocarmine staining (i-iv), fluorescein diacetate (FDA) staining (v-viii) and scanning microscopy, respectively (ix-xii). For acetocarmine and FDA scale bars: 50 μm. For SEM scale bars: 10 μm. b. Pollen development phenotyping using Hoechst 33342 DNA stain (i, iii, v, vii, ix, xi, xiii, xv, xvii and xix) and acetocarmine staning (ii, iv, vi, viii, x, xii, xiv, xvi, xviii and xx) in WT (i, ii, v, vi, ix, x, xiii, xiv, xvii and xviii) and iFRK3–3 mutant (iii, iv, vii, viii, xi, xii, xv, xvi, xix and xx) plants. PMC stage (i-iv). Tetrad stage (v-viii). Released microspores (ix-xii). Microspores after PMI (xiii-xvi). Mature pollen (xvii-xx). Scale bars: 20 μm