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Fig. 5 | BMC Plant Biology

Fig. 5

From: Molecular cytogenetic characterisation of Elytrigia ×mucronata, a natural hybrid of E. intermedia and E. repens (Triticeae, Poaceae)

Fig. 5

Mitotic metaphase chromosomes of nonaploid E. ×mucronata after sequential FISH and GISH experiments. a, c. and e GISH in plants 50–1, 50–7 and 41–5 (metaphase only with 62 chromosomes): Aegilops tauschii (digoxigenin, anti-DIG-FITC, yellow pseudocolour), Dasypyrum villosum (biotin, streptavidin-Cy3, blue pseudocolour), Hordeum bogdanii (biotin-streptavidin-Cy3, green pseudocolour), and Pseudoroegneria spicata (Cy5, red). b, d and f FISH in plants 50–1, 50–7 and 41–5 (metaphase only with 62 chromosomes): 5S rDNA (Cy5, red), 18S rDNA (digoxigenin, anti-DIG-FITC, green), and pSc119.2 (Cy3, white pseudocolour). After FISH, chromosomes are counterstained with DAPI. Different types of structural chromosomal alterations were indicated with numerals (1, 2, 5, 7). Scale bars = 10 μm

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BMC Plant Biology

ISSN: 1471-2229

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