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Fig. 3 | BMC Plant Biology

Fig. 3

From: Molecular cloning and functional analysis of 4-Coumarate:CoA ligase 4(4CL-like 1)from Fraxinus mandshurica and its role in abiotic stress tolerance and cell wall synthesis

Fig. 3

Regeneration of transgenic tobacco.A: (a) Tobacco plantlets up to 4–5 leaves were selected for infection; (b) Tobacco leaves infected with Agrobacterium tumefaciens were cultured in a sterilized culture medium; (c and e) Budding of tobacco leaves after induction in budding medium; (d) Tobacco that did not transform successfully after being kanamycin-screened died of whiteness; (f-h) Growth of tobacco roots in rooting medium; (b) GUS staining of transgenic tobacco seedlings, pistils, and stamens; (c) Transcript levels of Fm4CL-like 1 in different overexpression lines.

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