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Fig. 5 | BMC Plant Biology

Fig. 5

From: PbrSLAH3 is a nitrate-selective anion channel which is modulated by calcium-dependent protein kinase 32 in pear

Fig. 5

Electrical properties of PbrSLAH3 co-expressed with AtCPK21 in Xenopus laevis oocytes. a YFP fluorescence was emitted from Xenopus oocytes when PbrSLAH3 was co-expressed with AtCPK21, but no YFP fluorescence was observed after the injection of PbrSLAH3-YFP alone. b Representive macroscopic anion currents were recorded in different concentrations of external NO3− and Cl− when PbrSLAH3 was co-expressed with AtCPK21. Currents were recorded with 1.5-s voltage pulses ranging from − 180 mV to + 40 mV with 20-mV increments, followed by a 0.5-s voltage pulse to − 120 mV, the holding potential was 0 mV (n ≥ 5, means ± SE). c Steady state currents (Iss) plotted against the membrane voltages in the presence of different external NO3− concentrations (n ≥ 5, means ± SE)

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