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RNA-seq data reveals a coordinated regulation mechanism of multigenes involved in the high accumulation of palmitoleic acid and oil in sea buckthorn berry pulp

Abstract

Background

Sea buckthorn is a woody oil crop in which palmitoleic acid (C16:1n7, an omega-7 fatty acid (FA)) contributes approximately 40% of the total FA content in berry pulp (non-seed tissue). However, the molecular mechanisms contributing to the high accumulation of C16:1n7 in developing sea buckthorn berry pulp (SBP) remain poorly understood.

Results

We identified 1737 unigenes associated with lipid metabolism through RNA-sequencing analysis of the four developmental stages of berry pulp in two sea buckthorn lines, ‘Za56’ and ‘TF2–36’; 139 differentially expressed genes were detected between the different berry pulp developmental stages in the two lines. Analyses of the FA composition showed that the C16:1n7 contents were significantly higher in line ‘Za56’ than in line ‘TF2–36’ in the mid-late developmental stages of SBP. Additionally, qRT-PCR analyses of 15 genes involved in FA and triacylglycerol (TAG) biosynthesis in both lines revealed that delta9-ACP-desaturase (ACP-Δ9D) competed with 3-ketoacyl-ACP-synthase II (KASII) for the substrate C16:0-ACP and that ACP-Δ9D and delta9-CoA-desaturase (CoA-Δ9D) gene expression positively correlated with C16:1n7 content; KASII and fatty acid elongation 1 (FAE1) gene expression positively correlated with C18:0 content in developing SBP. Specifically, the abundance of ACP-Δ9D and CoA-Δ9D transcripts in line ‘Za56’, which had a higher C16:1n7 content than line ‘TF2–36’, suggests that these two genes play an important role in C16:1n7 biosynthesis. Furthermore, the high expressions of the glycerol-3-phosphate dehydrogenase (GPD1) gene and the WRINKLED1 (WRI1) transcription factor contributed to increased biosynthesis of TAG precursor and FAs, respectively, in the early developmental stages of SBP, and the high expression of the diacylglycerol O-acyltransferase 1 (DGAT1) gene increased TAG assembly in the later developmental stages of SBP. Overall, we concluded that increased ACP-Δ9D and CoA-Δ9D levels coupled with decreased KASII and FAE1 activity is a critical event for high C16:1n7 accumulation and that the coordinated high expression of WRI1, GPD1, and DGAT1 genes resulted in high oil accumulation in SBP.

Conclusion

Our results provide a scientific basis for understanding the mechanism of high C16:1n7 accumulation in berry pulp (non-seed tissue) and are valuable to the genetic breeding programme for achieving a high quality and yield of SBP oil.

Background

Sea buckthorn is a perennial shrub or small tree belonging to the genus Hippophae L. in the family Elaeagnaceae [1]. Sea buckthorn is one of the nutritionally and ecologically most important woody oil crops and has gained popularity worldwide due to berry pulp oil, which contains uniquely bioactive compounds [2]. SBP is a good source of edible oil. Bioactive oils (2–38%) with high levels of the uncommon fatty acid (FA) palmitoleic acid (approximately 40%) [2,3,4], carotenoids, flavonol glycosides, and tocopherol [5,6,7], can be extracted from the orange or red berry pulp.

Plant oils, mainly composed of triacylglycerols (TAGs), are an essential component of human diets. The common vegetable oils extracted from palm, soybean, rapeseed and sunflower contain five main FAs: palmitic (C16:0), stearic (C18:0), oleic (C18:1), linoleic (C18:2) and α-linolenic (C18:3) acids. In addition, many uncommon FAs are synthesized. Palmitoleic acid (cis-Δ9–16:1 or C16:1n7) is an important unusual omega-7 FA that has good oxidative stability for food production and provides human health benefits, such as increasing cell membrane fluidity and tissue regeneration, reducing inflammation, protecting the cardiovascular system, inhibiting oncogenesis, and treating hypertriglyceridaemia, with the additional benefits of decreasing low-density lipoprotein cholesterol and increasing high-density lipoprotein cholesterol levels [8,9,10,11]. C16:1n7 is rarely synthesized in common oil crops, and few plants are known to accumulate C16:1n7. Those that do include cat’s claw (Doxantha unguis-cati), which contains 64% C16:1n7 [12], followed by Tasmanian waratah (Telopea truncata) (45%) and macadamia nuts (Macadamia integrifolia) (30%) [13, 14]. However, the seeds of these plants are the source of C16:1n7, and no reports have addressed C16:1n7 biosynthesis and accumulation mechanisms in SBP and non-seed tissues.

FA biosynthesis and TAG accumulation in plant seeds involve multiple subcellular organelles and several enzymatic reactions [15]. The first step in FA biosynthesis is the conversion of acetyl coenzyme A (acetyl-CoA) into malonyl-acyl carrier protein (ACP) by acetyl-CoA carboxylase (ACC, EC 6.4.1.2 from KEGG database) and ACP-S-malonyl transferase (MAT). Malonyl-ACP is elongated to palmitoyl (C16:0)-ACP by the addition of two carbons at a time (six cycles of four reactions) via the fatty acid synthase (FAS) complex [16], and C16:0-ACP is the converted to C18:0-ACP and C16:1n7-ACP by 3-ketoacyl-ACP-synthase II (KAS II, EC 2.3.1.179) and delta-9 desaturase (Δ9D, EC 1.14.19.1), respectively. In plastids, these free FAs (C16:0, C16:1n7, C18:0, and C18:1n9) released from ACP by thioesterases are subsequently exported to the endoplasmic reticulum (ER) in the form of acyl-coenzyme A (acyl-CoA) esters [17, 18]. Acyl-CoAs can be further desaturated to C18:1n7, C18:2n6, and C18:3n3 in the ER by fatty acid elongation 1 (FAE1, EC 2.3.1.199) [8], fatty acid desaturase 2 (FAD2, EC 1.14.19.6), and fatty acid desaturase 3 (FAD3, EC 1.14.19.25), respectively, or utilized in the acylation of glyceraldehyde 3-phosphate (G3P), which is a primary substrate for TAG biosynthesis, and can be produced via a reaction catalysed by glycerol-3-phosphate dehydrogenase (GPD1, EC 1.1.1.8) during glycerol synthesis [19]. There are two TAG biosynthesis pathways. The Kennedy pathway is a major pathway for TAG biosynthesis in plants. In this process, the assembly of TAG from G3P and acyl-CoA involves three sequential acylations; the first two acylations of G3P are catalysed by glycerol-3-phosphate acyltransferase (GPAT, EC 2.3.1.15) and lysophosphatidic acid acyltransferase (LPAT, EC 2.3.1.51), respectively, followed by dephosphorylation by phosphatidate phosphatase (LPIN, EC 3.1.3.4) to produce DAG, and the third and final acylation is catalysed by diacylglycerol O-acyltransferase (DGAT, EC 2.3.1.20) to produce TAG [18]. In the second pathway, phosphatidylcholine (PC) is formed first, and its acyl residues are further desaturated. The choline phosphate residue is then liberated by hydrolysis, and the corresponding DAG is acylated. The phospholipid: diacylglycerol acyltransferase (PDAT, EC 2.3.1.158) results in the conversion of DAG to TAG [20]. Final TAG storage occurs in ER-derived oil bodies [19]. In addition, transcription factors (TFs) including WRINKLED1 (WRI1), LEAFY COTYLEDON (LEC), and FUSCA 3 (FUS3) positively regulate the expression of genes involved in FA biosynthesis and control plant oil levels [21,22,23]. The mechanism of normal FA biosynthesis and accumulation has been widely understood in model plant seeds in previous studies, such as Arabidopsis, Glycine max and Brassica napus [24, 25], but the regulatory mechanism of unusual omega-7 FAs (such as C16:1n7) biosynthesis and TAG accumulation in sea buckthorn, especially in non-seed tissue, is poorly understood.

To identify genes related to C16:1n7 biosynthesis and TAG accumulation in SBP, we first generated a comprehensive transcriptome of berry pulp in four developmental stages from two lines, ‘Za56’ and ‘TF2–36’, that displayed substantial changes in oil content and FA composition. We identified 16 sets of differentially expressed genes (DEGs) during the development of berry pulp and identified the transcriptional patterns of DEGs involved in lipid metabolism. Furthermore, expression analysis of key genes in FA biosynthesis and TAG accumulation revealed that the high expression of ACP-Δ9D and CoA-Δ9D coupled with the downregulation of KASII and FAE1 is a critical event for high C16:1n7 accumulation, and the coordinated high expression of GPD1 and WRI1 in the mid-early developmental stages and DGAT1 in the mid-late stages increased TAG assembly in SBP. These results provide a scientific basis for understanding the mechanism of C16:1n7 biosynthesis and accumulation in berry pulp (non-seed tissue) and are valuable to the genetic breeding programme for achieving a high quality and yield of SBP oil.

Oil extraction and analysis

Total oils were extracted from SBP as described previously [26]. Briefly, 0.3 g of dried pulp powder (m1) was homogenized in 6 mL chloroform-methanol (2:1, v/v) for 2 min. The mixtures were sonicated in an ultrasonic bath for 30 min, centrifuged and filtered. The solid residues were re-suspended in 4 mL chloroform-methanol (2:1, v/v), homogenized for 3 min and filtered. A volume of 1 mL 0.88% KCl was added to the combined filtrates, and the mixtures were mixed thoroughly by vortexing and were then centrifuged. The lower phase containing the purified oils (m2) was collected and evaporated under nitrogen. The oil content was calculated as follows: oil content (%) = m2/m1 × 100%.

Fatty acid methyl ester (FAME) and gas chromatography time-of-flight mass spectrometry (GC-TOF/MS) analyses of berry pulp

The FA composition was determined by FAMEs extraction with boron trifluoride in methanol as a catalyst according to previous studies [27, 28]. Briefly, 0.2 g of the dried powder samples was transferred into a glass test tube, followed by the addition of 2 mL n-hexane and 5 mL methanol-potassium hydroxide solution (1 M). The mixture was placed in a vibrating water bath at 60 °C for 30 min. After the reaction was complete, 10 mL boron trifluoride in methanol was added to the mixture, and the samples were left at 60 °C for 30 min. Two millilitres of saturated sodium chloride solution and 2 mL n-hexane were added, and FAMEs were then extracted by vigorous shaking for approximately 1 min. Following centrifugation, the aliquots were dried with anhydrous sodium sulfate, and the top layer was transferred into a vial, which was flushed with nitrogen; the vials were stored at − 20 °C until analysed by gas chromatography. The analyses were conducted in triplicate.

GC-TOF/MS analysis of FAMEs was performed on a Clarus 680 GC coupled to an AxION iQT TOF/MS system (PerkinElmer, Shelton, USA). The system was equipped with an Agilent J&W DB-23 capillary column (60 m × 0.25 mm × 0.25 μm). Helium was used as a carrier gas (flow rate: 1 mL·min− 1). The injector was operated in split mode (1:20). The inlet and transfer line temperatures were 230 °C and 215 °C, respectively. The temperature program was as follows: the initial temperature of 50 °C was raised by 15 °C·min− 1 to 200 °C, increased by 3 °C·min− 1 to 215 °C and finally increased to 230 °C for 10 min. For the detection of the mass spectrum, the temperature of the ion source was 230 °C. The operation parameters were an electron ionization (EI) ion source, electron energy of 70 eV, electron multiplier of 1.5 kV, solvent delay of 5 min, scanning range of 45–400 amu, and injection volume of 1 μL using the full-scan mode. FAs were identified by comparison with the retention times in the total ion chromatography (TIC) spectra and the mass-to-charge ratio (m/z) in the mass spectra of 37 FAME standards (Sigma-Aldrich, Steinheim, Germany). The FA composition was expressed as the weight percentage of each FA in the total FA content.

Total RNA extraction, cDNA library construction and RNA-seq analysis

Total RNA was isolated from berry pulp in four developmental stages using a TRIzol RNA Extraction Kit (Invitrogen, Carlsbad, CA, USA). Total RNA was quantified and qualified by 1% gel electrophoresis, a NanoDrop (Thermo Fisher Scientific Inc.) and an Agilent 2100 bioanalyser (Agilent Technologies, Palo Alto, CA, USA). RNA samples with an A260/A280 ratio between 1.9 and 2.1, an A260/A230 ratio between 2.0 and 2.5 and a RIN (RNA integrity number) of ≥8.0 were processed for further analysis.

Total RNA from three biological replicates of each sample was used for cDNA preparation. Next-generation sequencing library preparations were constructed according to the manufacturer’s protocol (NEBNext Ultra™ RNA Library Prep Kit for Illumina). mRNA enrichment, fragment interruption, adapter addition, size selection and PCR amplification were performed by GENEWIZ Inc. (Beijing, China), and RNA-seq was conducted by an Illumina HiSeq2500 system. Briefly, mRNA was purified from 3 μg total RNA from berry pulp at each developmental stage from the two lines using oligo (dT) magnetic beads. Next, using these short fragments as templates, first-strand cDNA synthesis was carried out with random primers. Third, the ends of double-stranded cDNA fragments were further modified with DNA polymerase. After the end-repair process and adapter ligation, the products were enriched by PCR to construct the final cDNA library. The cDNA library was examined using an Agilent 2100 bioanalyser [29]. Finally, the eight berry pulp samples, from the four developmental stages of the two lines, were sequenced.

Assembly and functional annotation of unigenes

Eight RNA-seq libraries were constructed, and de novo transcriptome libraries were assembled from all samples. Ambiguous nucleotides (N) and low-quality bases in raw reads were removed based on the Q20, Q30, N and GC parentages to obtain clean reads. De novo assembly was performed using Trinity software r2013-02-25 [30]. The total and average lengths of the assembled contigs were important criteria for assessing transcriptome quality. Unigenes were defined after removing redundant and short contigs from the assembly.

Reads were mapped against the assembled de novo transcriptome using Bowtie2 [31]. All unigenes from sea buckthorn were aligned using BLAST to identify homologous genes and to the non-redundant protein (nr), Clusters of Orthologous Groups (COG), Swiss-Prot (an annotated protein sequence database) and Kyoto Encyclopedia of Genes and Genomes (KEGG) databases [32] using Blastx algorithms with a threshold E-value of ≤10− 5. The unigenes were mapped to the KEGG metabolic pathway database to elucidate the complex biological behaviours of unigenes using KEGG Automatic Annotation Server (KASS) [33]. Using the COG database, orthologous gene products were classified, and the functions of unigenes were predicted.

Identification of DEGs involved in FA biosynthesis and TAG accumulation

DEGs were identified from the unigenes for digital gene expression profile analysis, which was performed with a modified procedure using a rigorous algorithm. The normalization and calculation of unigene expression was performed by the fragments per kilobase of exon model per million mapped fragments (FPKM) method. A p value of < 0.05 and an absolute value of the log2 (fold change) of > 1 were the thresholds for significant differences in gene expression.

The significant DEGs involved in FA biosynthesis and TAG accumulation were screened based on alignments against the lipid metabolism pathways in the KEGG database. We first compared transcriptome differences among the four developmental stages of pulp in sea buckthorn berries specifically by analysing the transcript profiles of multigenes (ACC, KAR, FATA, KASII, ACP-Δ9D, CoA-Δ9D, FAE1, FAD2, FAD3, GPD1, GPAT, LPAT, DGAT1, PDAT, and WRI1) associated with C16:1n7 biosynthesis and TAG accumulation in berry pulp.

qRT-PCR analysis

The RNA samples used for qRT-PCR analysis were the same as those used for the RNA-seq, oil content and FA composition analyses. The first-strand cDNA used for qRT-PCR was synthesized from RNAs using a PrimeScript RT reagent kit with gDNA Eraser (TaKaRa, Dalian, China).

Primer pairs were designed according to the selected multigenes coding for FA and TAG biosynthesis enzymes/proteins using Primer 5.0 software (Additional file 1: Table S1). The UBQ5 gene was used as a reference gene for quantitative gene expression [4]. The qRT-PCR reactions were performed in 96-well plates using an ABI 7500 Real-Time PCR system (Applied Biosystems, Foster, United States) and a SYBR Premix Ex Taq II Kit (TaKaRa, Dalian, China) according to the manufacturer’s instructions. qRT-PCR was carried out on three replicates per sample, and the relative expression level of each gene was calculated by the 2-ΔΔCt method [34].

Results

Characterization of oil content and FA composition during berry pulp development in sea buckthorn

Oil content and FA composition across four developmental stages (S1, S2, S3, and S4) of SBP lines, ‘TF2–36’ and ‘Za56’ (Fig. 1a), were explored. The oil contents in the developing berry pulp of line ‘TF2–36’ exhibited rapid accumulation from 9.31% at S1 stage to 35.42% at S3 stage and were significantly higher than those in line ‘Za56’ (4.84–8.48%). During S3 to S4, the SBP oil contents of both lines remained stable (Fig. 1b).

Fig. 1
figure 1

Phenotypic observations and oil content in the developing berry pulp of the two sea buckthorn lines ‘Za56’ and ‘TF2–36’. a The developmental progress of fruits from lines ‘Za56’ and ‘TF2–36’ (S1–S4). S1, S2, S3 and S4 indicate the developmental stage of berry pulp collected on July 6, July 28, August 19 and September 10, respectively. b Oil content was measured at four developmental stages during pulp development in lines ‘Za56’ and ‘TF2–36’. * indicate significant differences of data between the two lines at the same developmental stage at the level of 0.05

To reveal the FA composition in SBP, we performed GC-TOF/MS analyses to measure the FA composition at four developmental stages. Eleven and seven kinds of FAs were measured in the berry pulp from lines ‘Za56’ and ‘TF2–36’, respectively. C16:0, C16:1n7, C18:0, oleic (C18:1n9), vaccenic (C18:1n7), C18:2n6 and C18:3n3 were identified in both lines; four other FAs (myristic, pentadecanoic, hexadecadienoic and arachidic acids) were also detected in the berry pulp from line ‘Za56’ (Fig. 2a and Additional file 2: Table S2).

Fig. 2
figure 2

FA composition in SBP from lines ‘Za56’ and ‘TF2–36’ at four developmental stages. a Changes in the composition of various FAs in each line. b Comparison of five major FAs between two lines. The error bars indicate the standard deviations of three biological replicates. * indicate significant differences of FA composition between the two lines at the same developmental stages at the level of 0.05

Of the saturated fatty acids (SFAs), C16:0 was the most abundant in SBP, accounting for the highest (30.21% in line ‘Za56’ and 39.22% in line ‘TF2–36’) percentage of all FAs; furthermore, the C16:0 content was higher in line ‘TF2–36’ than in line ‘Za56’ at every stage (Fig. 2b). However, the C18:0 content in line ‘TF2–36’ were significantly lower than the C16:0 content in line ‘TF2–36’ and lower than the C18:0 content in line ‘Za56’ in developing berry pulp. The monounsaturated fatty acid (MUFA) (C16:1n7, C18:1n9, and C18:1n7) contents, accounting for 36.19–51.91% and 39.17–55.68% of all FAs in lines ‘Za56’ and ‘TF2–36’, respectively, were much higher than those of SFAs and polyunsaturated fatty acids (PUFAs) in each line for all four berry pulp developmental stages (Fig. 2a). In particular, the C16:1n7 content in both lines was predominant among the MUFAs, accounting for 13.31–41.30% in line ‘Za56’ and 12.57–39.03% in line ‘TF2–36’ and increasing during berry pulp development, and the C16:1n7 contents in line ‘Za56’ were higher than those in line ‘TF2–36’ from S2 to S4 (Fig. 2b and Additional file 2: Table S2). The contents of C18:1n9 in line ‘Za56’ (1.98–12.35%) were significantly lower than those in line ‘TF2–36’ (9.60–19.92%) and decreased from S1 to S4, but the C18:1n7 contents in line ‘Za56’ (7.34–10.53%) were higher than those in line ‘TF2–36’ (5.32–7.05%) (Fig. 2b). For the PUFAs, the contents of C18:2n6 and C18:3n3 decreased greatly from S1 to S3, and these two FAs were much more abundant in developing berry pulp from line ‘Za56’ than from line ‘TF2–36’ (Fig. 2a and Additional file 2: Table S2).

Sequencing, de novo transcriptome assembly and functional annotation

To obtain an overview of the sea buckthorn transcriptome in developing berry pulp and identify key candidate genes involved in lipid biosynthesis and accumulation in berry pulp (non-seed tissue), we performed transcriptome sequencing of berry pulps in four developmental stages (S1, S2, S3 and S4) from two sea buckthorn lines, ‘Za56’ and ‘TF2–36’. After removing adapter sequences, duplicated sequences, ambiguous reads and low-quality reads, a total of 397,823,136 clean reads were generated; 99.27–99.58% of bases had a Q of ≥20, and the reads had a 41.1–41.8% GC content (Table 1). The reads in each library were assembled into unigenes using the program Trinity. As a result, 323,881 unigenes were generated (≥ 200 bp), with an average size of 593.25 bp and an N50 length of 856 bp. In total, 98,829 unigenes (30.51%) were longer than 500 bp (Fig. 3). The mapping rates of clean reads mapped to unigenes were 54.84–66.25% (Table 1).

Table 1 Quality analysis of clean reads of RNA-seq
Fig. 3
figure 3

Length distributions of assembled unigenes

There were 79,413 unigenes (24.52%) annotated in the nr database, 69,924 unigenes (21.59%) annotated in the COG database, 24,456 unigenes (7.55%) annotated in the Gene Ontology (GO) database, 99,916 unigenes (30.85%) annotated in the Swiss-Prot database and 28,579 unigenes (8.82%) annotated in the KEGG database. Thus, a total of 122,305 (37.76%) unigenes were annotated in one or more public databases (Table 2 and Additional file 3: Table S3), and 15,997 unigenes were annotated in all four databases.

Table 2 Functional annotation of the sea buckthorn transcriptome

Some unigenes were annotated with multiple COG functions; therefore, 77,713 functional annotations were assigned to 25 COG clusters, which were grouped into four larger categories, namely, “cellular processes and signalling” (26,396 unigenes, 33.97%); “information storage and processing” (16,402, 21.11%); “metabolism” (22,202, 28.57%), containing 3153 unigenes involved in “lipid transport and metabolism” (Fig. 4a); and “poorly characterized” (12,713, 16.36%).

Fig. 4
figure 4

Functional classifications and annotation of unigenes. a COG classification: A, RNA processing and modifications; B, Chromatin structure and dynamics; C, Energy production and conversion; D, Cell cycle control, cell division, chromosome partitioning; E, Amino acid transport and metabolism; F, Nucleotide transport and metabolism; G, Carbohydrate transport and metabolism; H, Coenzyme transport and metabolism; I, Lipid transport and metabolism; J, Translation, ribosomal structure and biogenesis; K, Transcription; L, Replication, recombination and repair; M, Cell wall/membrane/envelope biogenesis; N, Cell motility; O, Posttranslational modification, protein turnover, chaperones; P, Inorganic ion transport and metabolism; Q, Secondary metabolites biosynthesis, transport and catabolism; R, General function prediction only; S, Function unknown; T, Signal transduction mechanisms; U, Intracellular trafficking, secretion, and vesicular transport; V, Defence mechanisms; W, Extracellular structures; Y, Nuclear structure; Z, Cytoskeleton. b KEGG classifications

The KEGG pathway database with the KAAS was used to predict the metabolic network of sea buckthorn. Using Blast2GO, all unigenes were mapped against the KEGG database, resulting in 28,579 unigenes grouped into 374 KEGG pathways and 43 pathway categories, which were grouped into six groups (Fig. 4b). The groups most enriched in unigenes were metabolism (17,572 unigenes). We focused on the “lipid metabolism” category, containing 1737 unigenes grouped into 17 pathways. The maximum number of unigenes involved in glycerophospholipid metabolism (ko00564) was 342; 222 unigenes were involved in glycerolipid metabolism (ko00561), and 141 unigenes were involved in FA biosynthesis (ko00061). Furthermore, 131 were related to the biosynthesis of unsaturated fatty acids (ko01040), 83 were in the FA elongation pathway (ko00062) and 189 were in the FA degradation pathway (ko00071).

Identification of DEGs involved in palmitoleic acid biosynthesis and TAG accumulation in SBP

To investigate the regulatory mechanism of FAs and TAG biosynthesis, especially the high accumulation of C16:1n7 in berry pulp (non-seed tissue), lipid metabolism pathways with unigenes identified in the SBP transcriptome were evaluated. We compared the normalized read counts of the unigenes and identified significant DEGs (p value < 0.05) among the developing SBP of lines ‘Za56’ and ‘TF2–36’. The significant DEGs were compared between the libraries of two different developmental stages (16 pairwise comparison groups, in Additional file 4: Table S4). A total of 30,530 downregulated and 27,430 upregulated DEGs were identified among the 16 pairwise comparison groups (Fig. 5). Compared to the number of DEGs in S1, the number of DEGs increased from S2 to S4. C16:1n7 and oils mostly accumulated in SBP during the period from S2 to S4 (Figs. 1b and 2b). In the S1 vs. S4 comparison for each line (Figs. 5a, b), we found the highest number of DEGs (3888 and 4079 in lines ‘Za56’ and ‘TF2–36’, respectively). A total of 3890, 3435, 3843 and 3665 DEGs were identified in the four pairwise comparison groups (S1, S2, S3 and S4, respectively) between lines ‘Za56’ and ‘TF2–36’ (Fig. 5c).

Fig. 5
figure 5

Numbers of upregulated and downregulated DEGs in the two sea buckthorn lines. a Numbers of DEGs in line ‘Za56’ by pairwise comparisons of four developmental stages. b Numbers of DEGs in line ‘TF2–36’ by pairwise comparisons of four developmental stages. c Numbers of DEGs between the two lines at the same developmental stage

Based on alignments against the KEGG pathway analysis, 139 DEGs (42 functional genes) were related to lipid metabolism (Table 3). Of these, 65 were involved in FA biosynthesis pathways in plastids, and 74 were associated with TAG biosynthesis (Additional file 5: Table S5). ACC is a key enzyme of FA precursor biosynthesis. The FPKMs of ACC (two homologous DEGs) were first upregulated from S2 to S3 and then downregulated from S3 to S4 in developing SBP of line ‘Za56’, and the total FPKM of line ‘Za56’ was much lower than that in line ‘TF2–36’ (Fig. 7a), which could provide more FA substrates for the assembly of TAG. To investigate the high level of C16:1n7 accumulation in SBP, we focused on several key enzymes related to C16:1n7 biosynthesis, which involves desaturation by two distinct classes of Δ9D genes, designated ACP-Δ9D and CoA-Δ9D (Fig. 6). The ACP-Δ9D gene catalyses the conversion of C16:0-ACP to C16:1n7-ACP, and the CoA-Δ9D gene catalyses the conversion of C16:0-CoA to C16:1n7-CoA, which have two and one homologous DEGs, respectively, and a total FPKM value of greater than 20 (Fig. 7a). The FPKMs of these three unigenes increased and then decreased, and the expression profiles of these genes correlated with the C16:1n7 content in both lines. In particular, the peak expression of these genes in line ‘Za56’, with a high C16:1n7 content, was higher than that in line ‘TF2–36’, with a low C16:1n7 content, during berry pulp development (Figs. 2b and 7a). Notably, two homologous DEGs of KASII, catalysing the conversion of C16:0-ACP to C18:0-ACP and/or C16:1n7-ACP to C18:1n7-ACP (Fig. 6), had downregulated expression patterns with low levels in the mid-late stages (Fig. 7a), and the C18:0 contents in both lines were less than 1% (Fig. 2b). Furthermore, the downregulation of FAE1, elongating of C16:1n7-CoA to C18:1n7-CoA, coordinated with low expression of KASII (Fig. 7a), which was responsible for the low contents of C18:1n7 (Fig. 2b). The transcription of FAD2 and FAD3 was also downregulated (Fig. 7a). These Δ9D and KASII genes regulated the reaction direction of C16:0-ACP conversion to C16:1n7-ACP and/or C18:0-ACP, suggesting a critically synergistic regulatory mechanism involved in high C16:1n7 accumulation in SBP.

Table 3 Differentially expressed genes involved in fatty acid and triacylglycerol biosynthesis
Fig. 6
figure 6

Schematic diagram representing palmitoleic acid biosynthesis and TAG accumulation in SBP. The bold arrows in red indicate the metabolic flux from C16:0-ACP to C16:1n7-TAG. The red (upregulation) and green (downregulation) boxes indicate the key genes in C16:1 biosynthesis and its accumulation in TAG. The violet arrows indicate the Kennedy pathways in TAG assembly. The blue arrows indicate the phosphatidylcholine acyl pathways in TAG assembly. The enzymes are shown in boxes and abbreviated as follow: ACC, acetyl-CoA carboxylase; KAS, 3-ketoacyl-ACP synthase (KAS I, KAS II, KAS III); KAR, 3-ketoacyl-ACP reductase; HAD, β-hydroxyacyl-ACP dehydrase; EAR, enoyl-ACP reductase; FATA, acyl-ACP thioesterase A; FATB, acyl-ACP thioesterase B; Δ9D, delta-9 desaturase; FAE1, fatty acid elongation 1; FAD2, fatty acid desaturase 2; FAD3, fatty acid desaturase 3; GPD1, glycerol-3-phosphate dehydrogenase; GPAT, glycerol-3-phosphate O-acyltransferase; LPAT, lysophosphatidic acid acyltransferase; LPIN, phosphatidate phosphatase; DGAT, diacylglycerol O-acyltransferase; plcC, phospholipase C; PDAT, phopholipid:diacyglycerol acyltransferase. The names of key intermediates are abbreviated as follows: G3P, glycerol-3-phosphate; LPA, lysophosphatidic acid; PA, phosphatidic acid; DAG, diacylglycerol; TAG, triacylglycerol

Fig. 7
figure 7

Heat maps of unigenes involved in FA biosynthesis (a) and TAG accumulation (b) in SBP. The expression value (in FPKM) for the unigenes during berry pulp development in both lines was log2 transformed, and the total FPKM value was greater than 20

TAG assembly is an important step in oil accumulation. G3P is a primary substrate, converted into TAG by three sequential acylations by GPAT, LPAT and DGAT (Fig. 6). Nine DEGs, including 3 GPD1, 2 GPAT, 1 LPAT, 2 LPIN, and 1 DGAT1 (the total FPKM of each transcript was greater than 20), were identified in the developing berry pulp transcriptome of sea buckthorn (Fig. 7b). GPD1, a rate-limiting enzyme in G3P synthesis, had the highest expression level in the mid-early stages (S1–S2), and the transcript levels in developing berry pulp from line ‘TF2–36’, with a high oil content, were higher than those in line ‘Za56’, with a low oil content (Fig. 1b). GPAT and LPAT had the highest transcript levels in S1, and the transcripts of these two genes in developing berry pulp of line ‘TF2–36’ were higher than those in line ‘Za56’. DGAT1, a rate-limiting enzyme in TAG assembly, had the highest expression level in the mid-late stages (S3–S4) and its transcript level was higher in line ‘TF2–36’ than in line ‘Za56’ (Fig. 7b). In addition, we identified two significant difference expression WRI1 (c141864_g1_i1 and c141864_g1_i2) TF in de novo transcriptome library of SBP, the FPKMs of WRI1 (c141864_g1_i1) in line ‘TF2–36’ berry pulp from S1 to S4 (30.3, 53.3, 31.2, and 10.7, respectively) were always higher than those in line ‘Za56’ (10.7, 8.2, 9.8, and 5.0, respectively). These results indicated that the high expression of the multigenes (GPD1, GPAT, LPAT, LPIN, DGAT1, and WRI1) contributed to TAG biosynthesis and accumulation.

qRT-PCR analysis reveals differential transcript abundances during berry pulp development in sea buckthorn

In plants, FA and oil accumulation in non-seed tissues involve several organelles and multiple enzymatic reactions, and there is no available genetic information on the regulatory mechanism of C16:1n7 and TAG biosynthesis in SBP (non-seed tissue). Based on previous studies [4, 18, 35] and the KEGG pathway analysis results (Additional file 5: Table S5), we selected 15 DEGs to determine by qRT-PCR whether the expression pattern during SBP development in lines ‘Za56’ and ‘TF2–36’ correlated with C16:1n7 biosynthesis and the changes in oil content. Nine candidate genes were involved in the FA biosynthesis pathway, namely, ACC (c135286_g1_i2), KAR (c119073_g1_i1), FATA (c141493_g1_i2), KAS II (c147281_g2_i3), FAE1 (c108825_g1_i1), ACP-Δ9D (c108934_g1_i3), CoA-Δ9D (c119361_g2_i1), FAD2 (c129094_g1_i1), and FAD3 (c146563_g1_i2). Five candidate genes, namely, GPD1 (c138230_g1_i1), GPAT (c131644_g1_i1), LPAT (c129657_g2_i2), DGAT1 (c144982_g1_i2), and PDAT (c133634_g3_i1), were involved in the TAG biosynthesis pathway (Fig. 6). One TF WRI1 (c141864_g1_i1) was associated with FA and oil biosynthesis-related genes expression and regulation. The expression levels of these 15 unigenes in S1, S2, S3, and S4 were mostly consistent between the qRT-PCR and RNA-seq experiments (Fig. 8), which indicated that the expression profiles in our transcriptomics results had a high confidence level.

Fig. 8
figure 8

qRT-PCR analysis of genes involved in C16:1n7 biosynthesis and TAG accumulation in lines ‘Za56’ and ‘TF2–36’ at four different developmental stages

ACP-Δ9D, CoA-Δ9D, KASII, and FAE1 were important coordinated regulatory genes related to C16:1n7 biosynthesis (Fig. 6). The relative expression levels of ACP-Δ9D and CoA-Δ9D first increased from S1 to S3 and then decreased in the berry pulp from both lines (Fig. 8). In particular, the peak expression of these two genes in line ‘Za56’, with a high C16:1n7 content, were higher than those in line ‘TF2–36’, with a low C16:1n7 content, in S3 (Fig. 2). However, KASII and FAE1 expression in both lines were significantly downregulated from S1 to S4, which corresponded to the decreasing trend in C18:0 and C18:1n7 contents (Fig. 2). The ACC expression in developing berry pulp from line ‘TF2–36’ was significantly higher than that in line ‘Za56’, and the peak KAR expression in line ‘TF2–36’ was higher than that in line ‘Za56’, with lower C16:0 contents. The expression of the FATA gene in line ‘Za56’ berry pulp was higher than that in line ‘TF2–36’ in S3 and S4. The downregulated expression of the FAD2 and FAD3 genes during berry pulp development in both lines was consistent with the decreasing contents of C18:2 and C18:3, respectively (Fig. 2).

GPD1 first increased at S1–S2 and then declined from S3 to S4 in developing berry pulp from both lines (Fig. 8). The expression levels of GPAT, LPAT, and PDAT in both lines decreased from S1 to S4. In developing berry pulps of line ‘TF2–36’ DGAT1 expression increased from S1 to S3 but increased from S2 to S4 in line ‘Za56’. WRI1 in line ‘TF2–36’ sharply increased at S1–S2 and then declined from S2 to S4, which was similar to the expression pattern of GPD1 in developing berry pulps of line ‘TF2–36’. The relative expression levels of GPD1, GPAT, LPAT, DGAT1, PDAT, and WRI1 in developing berry pulp from line ‘TF2–36’, with a high oil content, were higher in every stages than those in line ‘Za56’, with a low oil content (Fig. 1). Interestingly, the coordinated high expression of GPD1 and WRI1 in the mid-early developmental stages and DGAT1 in the mid-late stages contributed to increased biosynthesis of the TAG in SBP.

Discussion

Palmitoleic acid, an omega-7 FA, has numerous health benefits, such as protecting the cardiovascular system and inhibiting oncogenesis [8]. Due to the high value of and growing demand for omega-7 FAs, exploiting oleaginous resources and dissecting the mechanism of C16:1n7 biosynthesis and oil accumulation have been extensively pursued [12, 36,37,38]. Recently, sea buckthorn has gained popularity as a resource in the food and nutraceutical industries [1] because C16:1n7 is synthesized at very low levels in common oil crop seeds but can accumulate to 0.1–12% of all FAs in sea buckthorn seed oil [39, 40] and, more notably, accounts for 12–52% of all FAs in SBP oil [3, 41], with a ratio of MUFA to SFA of approximately 1:1 [28]. SBP oil with a high C16:1n7 content is a very balanced source of MUFAs for human health and nutrition, but the molecular mechanism of high C16:1n7 accumulation is still poorly understood. Therefore, considerable research efforts related to the exploration of C16:1n7 biosynthesis and TAG accumulation in SBP (non-seed tissue) are urgently needed. To explore whether transcriptional regulation is involved in controlling C16:1n7 biosynthesis and TAG accumulation in SBP, we analysed the dynamic patterns in oil content and FA composition, especially for C16:1n7, in developing berry pulps from lines ‘Za56’ and ‘TF2–36’ (Fig. 1). Of these two lines, line ‘Za56’ has a higher C16:1n7 content than that in line ‘TF2–36’. We carried out comparative transcriptomics analysis in the two lines at four berry pulp developmental stages and generated 183,235,418 and 214,587,718 clean reads for lines ‘Za56’ and ‘TF2–36’, respectively (Table 1). Bioinformatics analysis identified a total of 323,881 unigenes, 122,305 of which were annotated, in developing SBP. A total of 139 DEGs (42 functional genes) were found to be involved in lipid metabolism. Of these, 65 were involved in FA biosynthesis and 74 were associated with TAG accumulation (Table 3), suggesting that these DEGs regulate FA biosynthesis and TAG accumulation in SBP. The coordinated expression of genes involved in FA biosynthesis and TAG accumulation (ACC, KAR, FATA, KAS II, FAE1, ACP-Δ9D, CoA-Δ9D, FAD2, FAD3, GPD1, GPAT, LPAT, DGAT1, PDAT, and WRI1) contributes to the high levels of C16:1n7 in SBP oil. These results provide a scientific basis for understanding the mechanisms of C16:1n7 biosynthesis and accumulation in berry pulp (non-seed tissue) and are valuable to the genetic breeding programme for achieving a high quality and yield of SBP oil.

FA composition and oil content during berry pulp development in sea buckthorn

Because berry pulp and seed oils have unique bioactive compounds (FAs, phytosterols, carotenoids, and tocopherols), sea buckthorn berries have been used for hundreds of years in Russia and China for medicinal and nutritional purposes. Monounsaturated palmitoleic acid is rare in oil crops, such as soybean (relative C16:1n7 content = 0.14%), rapeseed (0.24%), and olive (0.73) [42], and a few rare plants, such as Kermadecia sinuate (70%), Doxantha unguis-cati (64%), and Tasmanian waratah (45%), are known to accumulate C16:1n7 in the seeds [8]. However, unlike these plants that accumulate C16:1n7 in the seeds, SBP oil has a C16:1n7 content of approximately 40% suitable for commercial extraction [2,3,4] In fact, approximately 40% of plant oils are extracted from non-seed tissues [43], and different amounts of C16:1n7 can be synthesized in different non-seed tissues, for example, 5.36% in the pericarp of bayberry [44], 0.41% in the leaves of tobacco [45], and trace amounts in the mesocarp of oil palm [17] and Chinese tallow [46]. Regarding C16:1n7 biosynthesis, the underlying mechanistic details are not yet well understood, particularly in non-seed tissue with high C16:1n7 accumulation.

Sea buckthorn is a unique species with a high accumulation of C16:1n7 in non-seed tissue (berry pulp) [8, 47], and the molecular mechanism of C16:1n7 biosynthesis and accumulation in berry pulp has not yet been reported. In this study, we measured the composition of the main FAs, especially C16:1n7, and the oil contents in developing berry pulp from the sea buckthorn lines ‘Za56’ (hybrid progeny of ssp. mongolica and ssp. sinensis) and ‘TF2–36’ (ssp. mongolica), with a closed genetic relationship [48]. The C16:1n7 content was the highest among all FAs in mature berry pulp from both line ‘Za56’ (41%), with a low oil content (4.8–8.5%), and line ‘TF2–36’ (39%), with a high oil content (9.3–35.4%) (Figs. 1 and 2). The contents of C16 FAs (C16:0 and C16:1n7) in mature pulp (S4) were significantly higher than those in immature pulp (S1) from both lines, while the contents of C18 FAs (C18:0, C18:1n9, C18:2, and C18:3) sharply decreased during berry pulp development (Fig. 2). As reported previously, the content of C16 FAs sharply decreased from 28.77 to 6.06% in developing seeds of sea buckthorn line ‘SJ1’, and C18 FAs accumulated to high levels in mature seeds (93.94%), which were much higher than those in immature seeds (71.23%) [35]. We also found similar accumulation patterns of C16 and C18 FAs between the two tissues in one line, ‘XE3’ [2]. The results of comparative analysis among these selected samples indicate that the metabolic flux from C16:0 to C16:1n7 or C18:0 plays an important role in determining the composition of FAs and TAG and provide the critical basis for understanding the biosynthesis and accumulation mechanism of berry pulp (non-seed tissue) with a high oil content.

Coordinated regulation of multigenes contributes to C16:1n7 accumulation in SBP

The sea buckthorn transcriptome libraries for developing berry pulps were first constructed, and 65 DEGs involved in FA biosynthesis and 74 DEGs associated with TAG accumulation were revealed (Table 3). We focused on C16:1n7 biosynthesis and the associated TAG accumulation pathway (Fig. 6) to confirm the gene expression patterns of related key enzymes in developing berry pulp from sea buckthorn lines ‘Za56’ and ‘TF2–36’ (Figs. 7 and 8).

The key steps in the biosynthesis and accumulation of C16:1n7 and TAG in SBP are outlined in red in Fig. 6. Three major biosynthesis events occur during the production of C16:1n7. The first involves the biosynthesis of C16:0-ACP and/or C16:1n7-ACP in plastids and the export of these molecules to the cytosol. The second involves the formation of C16:0-CoA and/or C16:1n7-CoA and the transfer of these products to the ER, where C16:0-CoA can be further converted to C16:1n7-CoA. The third involves the incorporation of nascent C16:1n7 into TAGs, which subsequently accumulate in oil bodies. Previous studies revealed that the Δ9D enzyme catalyses the desaturation of C16:0 to form C16:1n7, and some Δ9D enzymes can also convert C18:0 to C18:1n9 [8]. Numerous Δ9D genes from various plants have been cloned and characterized so far [37, 49, 50], and many studies have verified the expression of Δ9D genes capable of converting C16:0 to C16:1n7 in transgenic plants, including Arabidopsis, Nicotiana tabacum, Glycine max, and Brassica napus [12, 36, 38, 51]. In this study, we discovered two distinct classes of Δ9D enzymes, designated ACP-Δ9D (soluble) and CoA-Δ9D (membrane-bound), in SBP. The higher levels of ACP-Δ9D (1–53.2) and CoA-Δ9D (1–5.5) expression in developing SBP from line ‘Za56’ than in line ‘TF2–36’ (1–46.4 and 1–2.8, respectively) (Fig. 8) were consistent with the higher accumulation of C16:1n7 in ‘Za56’ (13.3–41.3%) than in ‘TF2–36’ (12.6–39.0%) (Fig. 2b and Additional file 2: Table S2). Gao et al. [52] found that the ACP-Δ9D enzyme desaturated C16:0 to C16:1n7 with high activity and that the C16:1n7 content in transgenic tobacco leaves overexpressing the Macfadyena unguis-cati ACP-Δ9D gene significantly increased to 16.4–20.4% compared to that in wild-type leaves (< 0.4%). Cytosolic CoA-Δ9D can also function in plastids of plant cells; seed-specific expression of a yeast CoA-Δ9D in soybean seeds resulted in (10.5%) plastid C16:1 n7 production higher than that in wild-type soybean seeds (< 0.1%) [36]. Furthermore, co-expression of a castor ACP-Δ9D gene in plastids and two fungal (Stagonospora nodorum and Aspergillus nidulans) CoA-Δ9D genes in the ER can increase the C16:1n7 content from 1.9 to 43% in transgenic Arabidopsis seeds [51].

However, we found that the coordinated upregulation of ACP-Δ9D and CoA-Δ9D and downregulation of KASII and FAE1 was critical in SBP oil biosynthesis to achieve high C16:1n7 accumulation (Figs. 6 and 8). During the development of SBP, an increasing total C16 FA (C16:0 and C16:1n7) content coupled with a sharp decline in C18 FA content; the content of C16 FAs was significantly higher than that of C18 FAs in S2–S4 (Additional file 2: Table S2). The length and desaturation of FA carbon chains are regulated by the activity of KASII, FAE1, Δ9D, FAD2, and FAD3. KASII competes with ACP-Δ9D for substrate and elongates the majority of C16:0-ACP to C18:0-ACP and/or C16:1n7-ACP to C18:1n7-ACP; FAE1 is responsible for the extraplastidial elongation of C16:1n7-CoA to C18:1n7-CoA [8]. Therefore, KASII was strongly downregulated in developing SBP, resulting in an increased accumulation of C16:0-ACP, which is then desaturated by ACP-Δ9D to produce C16:1n7-ACP. Furthermore, the higher CoA-Δ9D activity coordinated with the downregulation of FAE1 led to the increasing accumulation of C16:1n7-CoA. Previous studies revealed that high KAS II expression was consistent with the high proportion of C18 FAs in peony seeds [53] and that the suppression of KAS II by short hairpin RNAi led to an increase in C16:0 accumulation in palm seed oil [54]. Expression of castor ACP-Δ9D gene in wild-type Arabidopsis seeds increased C16:1n7 accumulation from 0.1 to 1.6%, yielding a total of 14.4% omega-7 FA, furthermore, the overexpression of ACP-Δ9D in a fab1 (KASII) mutant increased the accumulation of C16:1n7 to 23.5%, and the expression of ACP-Δ9D in the fab1 fae1 double mutant resulted in an increase in C16:1n7 to 26.2% in transgenic seeds [51].

The downregulation of KASII correlated with the decreased content of C18:0, which was only approximately 0.8% in mature berry pulp (S4). Moreover, the content of C18 unsaturated fatty acids (UFAs) (C18:1n9, C18:1n7, C18:2n6, and C18:3n6) also sharply declined (Figs. 2 and 8). Although ACP-Δ9D also catalyses the conversion of C18:0-ACP to C18:1n9-ACP [55], storage lipid synthesis is restricted by the supply of precursors [56]. Therefore, the sharp decline in C18:1n9 content in developing SBP was due to a lack of the C18:0-ACP substrate (Fig. 2). However, the decreased contents of C18:2n3 and C18:3n6 in berry pulp from both sea buckthorn lines were associated with the downregulation of the FAD2 and FAD3 genes, respectively (Figs. 2 and 8). Interestingly, in our previous study, we found that the high accumulation of C18 UFAs (88% of the total FAs) in sea buckthorn seed oil was due to low expression of the CoA-Δ9D gene coordinated with high expression of the KAS II, ACP-Δ9D, FAD2, and FAD3 genes [28]. These results are significant for understanding the mechanism of high C16:1n7 accumulation in SBP (non-seed tissue) and provide a scientific basis for enhancing C16:1n7 biosynthesis in non-seed tissues by means such as overexpressing Δ9D, downregulating KASII, co-expressing ACP-Δ9D in plastids and CoA-Δ9D in the ER, and optimizing the metabolic flux toward TAG assembly.

Source and sink genes control TAG biosynthesis and assembly in SBP

The glycerol or G3P supply is co-limiting for lipid synthesis; attempts to improve TAG synthesis solely by increasing the FA supply may not be successful and will need approaches that lead to a simultaneous increase in both FAs and G3P [19]. GPD1 and DGAT are described as the ‘source’ and ‘sink’ genes, respectively, in TAG biosynthesis; regulating the expression of these two genes can increase oil content in plant tissues [57]. GPD1 is a rate-limiting enzyme in lipid synthesis [58], and G3P, a primary substrate for TAG biosynthesis, can be produced via a reaction catalysed by GPD1 during glycerol synthesis [59]. DGAT and PDAT catalyse the conversion of DAG to TAG [20, 60]. In addition, WRI1 TF regulates the expression of genes involved in FA and TAG biosynthesis [21]. In the present study, we confirmed the differential expression levels of genes involved in TAG biosynthesis and accumulation in the developing pulp of two sea buckthorn lines, ‘Za56’ and ‘TF2–36’. The GPD1, GPAT, LPAT, DGAT1, and PDAT genes were more highly expressed in line ‘TF2–36’ (with a high pulp oil content) than in line ‘Za56’ (with a low pulp oil content) by qRT-PCR (Fig. 8), consistent with the data for the oil contents in the berry pulp from the two lines (Fig. 1). However, the expressions of GPD1 and DGAT1 genes had obviously upregulated trend, and GPAT, LPAT, and PDAT expressions always decreased during the development stages of both lines (Fig. 8). Therefore, we concluded that the coordinated high expression of GPD1 and DGAT1 genes resulted in high oil accumulation in SBP.

The peak GPD1 expression in lines ‘Za56’ and ‘TF2–36’ appeared during the period of rapid oil biosynthesis (mid-early stages), and the highest oil contents in the pulp were 8.48 and 35.42%, respectively, in S3 (Fig. 1), which was after the stage of peak GPD1 expression (S2) (Fig. 8). Therefore, the high expression of the GPD1 gene contributed to increased synthesis of the TAG precursor G3P in the early stages of berry pulp development. In planta feeding, glycerol leads to increased levels of G3P and TAG in developing Brassica napus seeds, and the oil content peak lagged behind that of G3P for 15 d [19]. In addition, Vigeolas et al. [61] found that a twofold overexpression of the yeast gene coding for GPD1 led to a three- to fourfold increase in the level of G3P in transgenic Brassica napus seeds, resulting in a 40% increase in oil content, with the protein content remaining substantially unchanged. Overexpression of maize GPD1 remarkably enhanced the G3P production and the salinity/osmotic stress tolerance in transgenic Arabidopsis plants, and the transgenic lines showed no aberrant phenotype under normal growth conditions, no matter in the vegetative growth phase or reproductive developmental stages [62]. Overexpression of the yeast GPD1 increased Camelina sativa seed oil content about 5.4% but cause no change in seed protein content and no effect on seed germination and seedling growth [63].

The peak DGAT1 expression in berry pulp from line ‘TF2–36’ (with a high oil content) appeared during the period of stable oil accumulation (mid-late stages) and was significantly higher than that in line ‘Za56’ (with a low oil content) in S3. Therefore, the high expression of DGAT1 catalysed the robust assembly of acyl-CoA FAs and DAG to form TAG in the late stages of berry pulp development. Li et al. [24] found that DGAT1 appears to be a major enzyme for seed oil accumulation in Arabidopsis and soybeans. Overexpression of DGAT cDNA in wild-type Arabidopsis seeds increased the oil content by 11–28% [64]. In addition, seed-specific overexpression of AtDGAT1 in Brassica juncea seed improved the oil content significantly, with a maximum oil content increase of 8.3% in the transgenic plants compared to that in the wild-type plants [65]. In addition, we examined the expression profiles of the GPD1 and DGAT1 genes in seeds and berry pulp tissues of one sea buckthorn line, ‘XE3’, and found that these two genes had higher expression in pulp (with an oil content of 36.90%) than in seeds (14.15%) [66].

Metabolic engineering strategies for increasing plant lipids can be described as the surprising synergy when ‘Push’ approach (up-regulation of substrate biosynthesis by WRI1) and a ‘Pull’ approach (increasing TAG assembly by DGAT) are combined [67]. This strategy corresponded to the coordinated high expression of source gene (GPD1) and sink gene (DGAT) contributes to increase oil content in plant tissues [57]. In developing SBP, we found similar expression patterns between WRI1 and GPD1, and their expressions of line ‘TF2–36’ were higher than those in line ‘Za56’ with a sharp increase in the mid-early stages (Fig. 8). The coordinated high expressions of WRI1 and GPD1 in line ‘TF2–36’, with a high oil content, suggested to play a major role in the high levels of storage oils in SBP. Overexpression of the WRI1 in Arabidopsis and maize yielded higher TAG levels in seed [68, 69] while an Arabidopsis wri1 knockout mutant exhibit reduced seed oil content [70]. Vanhercke et al. [67] found that overexpression of the Arabidopsis WRI1 gene led to a 22-fold increase TAG levels in transgenic Nicotiana benthamiana dry leave, and the C16:1 content increased from 0.4 to 0.9%. Furthermore, when both the Arabidopsis WRI1 and DGAT1 genes were co-infiltrated (push and pull approach), a significant synergistic effect on leaf oil accumulation was observed with TAG levels increasing almost 100-fold and making up 2.48% of the leaf dry weight. In addition, Camelina sativa plants co-expressing a yeast cytosolic GPD1 and the Arabidopsis DGAT1 genes exhibited up to 13% higher seed oil content compared to wild-type plants, and these coexpressing lines showed significantly higher seed oil yields than the plants expressing GPD1 and DGAT1 alone [63]. Therefore, combining the overexpression of lipid biosynthetic genes, WRI1, GPD1, and DGAT1, appears to be a positive strategy to achieve a synergistic effect on the flux through the TAG biosynthesis pathway, and thereby further increase the oil yield. This finding suggests that the coordinated high expression of WRI1 and GPD1 in the mid-early stages and DGAT1 genes in the mid-late stages resulted in TAG assembly and oil accumulation in SBP.

Conclusion

Palmitoleic acid (an omega-7 FA) is rarely synthesized in common oil crops [8] but is an essential unusual FA that has a wide range of biological activities and benefits to human health [9,10,11]. SBP is a good source of bioactive edible oil (2–38%) with a high level of C16:1n7 (approximately 40%), and the global demand for sea buckthorn pulp oil is increasing [1,2,3,4]. However, the regulatory mechanism of C16:1n7 biosynthesis and TAG accumulation in SBP is poorly understood. In this study, we first generated a comprehensive transcriptome of berry pulp from two lines, ‘Za56’ and ‘TF2–36’, in four developmental stages. Oils and C16:1n7 predominantly accumulated in mature berry pulp stages, and the C16:1n7 contents in developing berry pulp from line ‘Za56’ (with a low oil content) were higher than those in line ‘TF2–36’ (with a high oil content). We identified 139 DEGs involved in lipid metabolism between the different developmental stages of berry pulp in the two lines. Furthermore, expression analysis of some key genes involved in FA biosynthesis and TAG assembly revealed that high expression of the ACP-Δ9D and CoA-Δ9D genes coordinated with downregulation of the KASII and FAE1 genes is a critical event for high C16:1n7 accumulation and that the coordinated high expression of GPD1, WRI1, and DGAT1 accelerated TAG accumulation in SBP. These results provide the first scientific basis for understanding the mechanism of C16:1n7 biosynthesis and accumulation in SBP (non-seed tissue) and are valuable to the genetic breeding programme for achieving a high quality and yield of SBP oil with a high level of C16:1n7.

Methods

Plant materials

Samples were collected from cultivated Hippophae L. trees (lines ‘Za56’ and ‘TF2–36’) growing in Institute of Berries at Heilongjiang Academy of Agricultural Sciences (voucher No. 02901, identified by Jin-you Shan and deposited at HAAS), Suiling county, Heilongjiang, China (47°14′12.3“ north latitude, 127°05’39.9” east longitude) under natural climate conditions. The orchard had a mean annual rainfall of 570.6 mm, a mean annual temperature of 2.0 °C, a mean annual evaporation capacity of 1242.5 mm and an effective accumulative temperature of 2460.4 °C [71]. The collection of all samples completely complies with local and national legislation permission. Line ‘Za56’ was selected from seedlings of the hybrid progeny of ssp. mongolica (♀) and ssp. sinensis (♂), and line ‘TF2–36′ was selected from seedlings of ssp. mongolica. The molecular marker-based genetic similarity of these two lines is 0.752 [48]. The three trees of each line selected as biological triplicates for fruit collection were cutting seedlings. The developing fruits of lines ‘Za56’ and ‘TF2–36′ were collected on July 6 (S1), July 28 (S2), August 19 (S3) and September 10 (S4), 2015. These samples were immediately frozen in liquid nitrogen and stored at − 80 °C until use. Eight RNA sequencing (RNA-seq) transcriptome libraries were constructed based on the developmental berry pulp from these two lines.

Abbreviations

ACC:

Acetyl-CoA carboxylase

ACP:

Acyl carrier protein

ACP-Δ9D:

Delta9-ACP desaturase

CoA-Δ9D:

Delta9-CoA desaturase

DEG:

Differentially expressed gene

DGAT1:

Diacylglycerol O-acyltransferase 1

ER:

Endoplasmic reticulum

FA:

Fatty acid

FAD2:

Fatty acid desaturase 2

FAD3:

Fatty acid desaturase 3

FAE1:

Fatty acid elongation 1

FAME:

Fatty acid methyl ester

FATA:

Fatty acyl-ACP thioesterase A

FPKM:

Fragments per kilobase of exon model per million mapped fragments

G3P:

Glyceraldehyde 3-phosphate

GC-TOF/MS:

Gas chromatography time-of-flight mass spectrometry

GPAT:

Glycerol-3-phosphate acyltransferase

GPD1:

Glycerol-3-phosphate dehydrogenase

KAR:

3-ketoacyl-ACP reductase

KAS II:

3-ketoacyl-ACP synthase II

LPAT:

Lysophosphatidic acid acyltransferase

MUFA:

Monounsaturated fatty acid

PDAT:

phospholipid diacylglycerol acyltransferase

PUFA:

Polyunsaturated fatty acid

SFA:

Saturated fatty acid

TAG:

Triacylglycerol

TIC:

Total ion chromatography

UBQ5:

Ubiquitin 5

WRI1:

WRINKLED1

References

  1. Ruan CJ, Rumpunen K, Nybom H. Advances in improvement of quality and resistance in a multipurpose crop: sea buckthorn. Crit Rev Biotechnol. 2013;33(2):126–44.

    Article  CAS  PubMed  Google Scholar 

  2. Ding J, Ruan CJ, Guan Y, Shan JY. Comparison of oil contents and fatty acid composition between developing sea buckthorn pulp and seed. China Oil and Fats. 2017;42(5):140–4.

    Google Scholar 

  3. Yang B, Kallio H. Fatty acid composition of lipids in sea buckthorn (Hippophae rhamnoides L.) berries of different origins. J Agric Food Chem. 2001;49(4):1939–47.

    Article  CAS  PubMed  Google Scholar 

  4. Fatima T, Snyder CL, Schroeder WR, Cram D, Datla R, Wishart D, et al. Fatty acid composition of developing sea buckthorn (Hippophae rhamnoides L.) berry and the transcriptome of the mature seed. PLoS One. 2012;7(4):e34099.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  5. Li W, Ruan CJ, Teixeira da Silva JA, Guo H, Zhao CE. NMR metabolomics of berry quality in sea buckthorn (Hippophae L.). Mol Breeding. 2013;31(1):57–67.

    Article  Google Scholar 

  6. Teleszko M, Wojdylo A, Rudzinska M, Oszmianski J, Golis T. Analysis of lipophilic and hydrophilic bioactive compounds content in sea buckthorn (Hippophae rhamnoides L.) berries. J Agric Food Chem. 2015;63(16):4120–9.

    Article  CAS  PubMed  Google Scholar 

  7. Gornas P, Misina I, Krasnova I, Seglina D. Tocopherol and tocotrienol contents in the sea buckthorn berry beverages in Baltic countries: impact of the cultivar. Fruits. 2016;71(6):399–405.

    Article  Google Scholar 

  8. Wu YM, Li RZ, Hildebrand DF. Biosynthesis and metabolic engineering of palmitoleate production, an important contributor to human health and sustainable industry. Prog Lipid Res. 2012;51(4):340–9.

    Article  CAS  PubMed  Google Scholar 

  9. Bernstein AM, Roizen MF, Martinez L. Purified palmitoleic acid for the reduction of high-sensitivity C-reactive protein and serum lipids: a double-blinded, randomized, placebo controlled study. J Clin Lipidol. 2014;8(6):612–7.

    Article  PubMed  Google Scholar 

  10. Yang T, Yu Q, Xu W, Li DZ, Chen F, Liu A. Transcriptome analysis reveals crucial genes involved in the biosynthesis of nervonic acid in woody Malania oleifera oilseeds. BMC Plant Biol. 2018;18:247.

    Article  PubMed  PubMed Central  Google Scholar 

  11. Olas B. Sea buckthorn as a source of important bioactive compounds in cardiovascular diseases. Food Chem Toxicol. 2016;97:199–204.

    Article  CAS  PubMed  Google Scholar 

  12. Bondaruk M, Johnson S, Degafu A, Boora P, Bilodeau P, Morris J, et al. Expression of a cDNA encoding palmitoyl-acyl carrier protein desaturase from cat's claw (Doxantha unguis-cati L.) in Arabidopsis thaliana and Brassiea napus leads to accumulation of unusual unsaturated fatty acids and increased stearic acid content in the seed oil. Plant Breed. 2007;126(2):186–94.

    Article  CAS  Google Scholar 

  13. Vickery JR. The fatty acid composition of seed oils of proteaceae: a chemotaxonomic study. Phytochemistry. 1971;10(1):123–30.

    Article  CAS  Google Scholar 

  14. Gummeson PO, Lenman M, Lee M, Singh S, Stymne S. Characterisation of acyl-ACP desaturases from Macadamia integrifolia Maiden & Betche and Nerium oleander L. Plant Sci. 2000;154:53–60.

    Article  CAS  PubMed  Google Scholar 

  15. Bates PD, Browse J. The significance of different diacylglycerol synthesis pathways on plant oil composition and bioengineering. Front Plant Sci. 2012;3:147.

    Article  PubMed  PubMed Central  Google Scholar 

  16. Xia EH, Jiang JJ, Huang H, Zhang LP, Zhang HB, Gao LZ. Transcriptome analysis of the oil-rich tea plant, Camellia oleifera, reveals candidate genes related to lipid metabolism. PLoS One. 2014;9(8):e104150.

    Article  PubMed  PubMed Central  Google Scholar 

  17. Bourgis F, Kilaru A, Cao X, Ngando-Ebongue GF, Drira N, Ohlrogge JB, et al. Comparative transcriptome and metabolite analysis of oil palm and date palm mesocarp that differ dramatically in carbon partitioning. Proc Natl Acad Sci U S A. 2011;108(44):12527–32.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  18. Zhang QY, Yu R, Xie LH, Rahman MM, Kilaru A, Niu LX, et al. Fatty acid and associated gene expression analyses of three tree peony species reveal key genes for α-linolenic acid synthesis in seeds. Front Plant Sci. 2018;9:106.

    Article  PubMed  PubMed Central  Google Scholar 

  19. Vigeolas H, Geigenberger P. Increased levels of glycerol-3-phosphate lead to a stimulation of flux into triacylglycerol synthesis after supplying glycerol to developing seeds of Brassica napus L. in planta. Planta. 2004;219(5):827–35.

    Article  CAS  PubMed  Google Scholar 

  20. Li RZ, Yu KS, Hildebrand DF. DGAT1, DGAT2 and PDAT expression in seeds and other tissues of epoxy and hydroxy fatty acid accumulating plants. Lipids. 2010;45(2):145–57.

    Article  PubMed  Google Scholar 

  21. Adhikari ND, Bates PD, Browse J. WRINKLED1 rescues feedback inhibition of fatty acid synthesis in hydroxylase-expressing seeds. Plant Physiol. 2016;171(1):179–91.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  22. Manan S, Ahmad MZ, Zhang YG, Chen BB, Haq BU, Yang JH, et al. Soybean LEC2 regulates subsets of genes involved in controlling the biosynthesis and catabolism of seed storage substances and seed development. Front Plant Sci. 2017;8:1604.

    Article  PubMed  PubMed Central  Google Scholar 

  23. Elahi N, Duncan RW, Stasolla C. Decreased seed oil production in FUSCA3 Brassica napus mutant plants. Plant Physiol Biochem. 2015;96:222–30.

    Article  CAS  PubMed  Google Scholar 

  24. Wiberg E, Banas A, Stymne S. Fatty acid distribution and lipid metabolism in developing seeds of laurate-producing rape (Brassica napus L.). Planta. 1997;203(3):341–8.

    Article  CAS  PubMed  Google Scholar 

  25. Clemente TE, Cahoon EB. Soybean oil: genetic approaches for modification of functionality and total content. Plant Physiol. 2009;151(3):1030–40.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  26. Ding J, Ruan CJ, Shan JY, Guan Y. Expression of key genes involved in lipid biosynthesis and accumulation during seeds formation and development in Hippophae rhamnoides. Acta Bot Boreal -Occident Sin. 2016;36(8):1642–7.

    Google Scholar 

  27. Sanchez-Salcedo EM, Sendra E, Carbonell-Barrachina AA, Martinez JJ, Hernandez F. Fatty acids composition of Spanish black (Morus nigra L.) and white (Morus alba L.) mulberries. Food Chem. 2016;190:566–71.

    Article  CAS  PubMed  Google Scholar 

  28. Ding J, Ruan CJ, Guan Y, Wu LR, Shan JY. Coordinated regulation mechanism of multigenes involved in high accumulation of C18 unsaturated fatty acids in sea buckthorn seed. Acta Bot Boreal -Occident Sin. 2017;37(6):1080–9.

    Google Scholar 

  29. Li GL, Qian H. Transcriptome using Illumina sequencing reveals the traits of spermatogenesis and developing testes in Eriocheir sinensis. PLoS One. 2017;12(2):e0172478.

    Article  PubMed  PubMed Central  Google Scholar 

  30. Grabherr MG, Haas BJ, Yassour M, Levin JZ, Thompson DA, Amit I, et al. Full-length transcriptome assembly from RNA-Seq data without a reference genome. Nat Biotechnol. 2011;29(7):644–52.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  31. Langmead B, Salzberg SL. Fast gapped-read alignment with bowtie 2. Nat Methods. 2012;9(4):357–9.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  32. Tatusov RL, Natale DA, Garkavtsev IV, Tatusova TA, Shankavaram UT, Rao BS, et al. The COG database: new developments in phylogenetic classification of proteins from complete genomes. Nucleic Acids Res. 2001;29(1):22–8.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  33. Kanehisa M, Goto S, Kawashima S, Okuno Y, Hattori M. The KEGG resource for deciphering the genome. Nucleic Acids Res. 2004;32:D277–80.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  34. Livak KJ, Schmittgen TD. Analysis of relative gene expression data using real-time quantitative PCR and the 2-ΔΔCt method. Methods. 2001;25:402–8.

    Article  CAS  PubMed  Google Scholar 

  35. Ding J, Ruan CJ, Guan Y, Krishna P. Identification of microRNAs involved in lipid biosynthesis and seed size in developing sea buckthorn seeds using high-throughput sequencing. Sci Rep. 2018;8:4022.

    Article  PubMed  PubMed Central  Google Scholar 

  36. Xue JA, Mao X, Yang ZR, Wu YM, Jia XY, Zhang L, et al. Expression of yeast acyl-CoA-∆9 desaturase leads to accumulation of unusual monounsaturated fatty acids in soybean seeds. Biotechnol Lett. 2013;35(6):951–9.

    Article  CAS  PubMed  Google Scholar 

  37. Troncoso-Ponce MA, Barthole G, Tremblais G, To A, Miquel M, Lepiniec L, Bauda S. Transcriptional activation of two delta-9 palmitoyl-ACP desaturase genes by MYB115 and MYB118 is critical for biosynthesis of omega-7 monounsaturated fatty acids in the endosperm of Arabidopsis seeds. Plant Cell. 2016;28(10):2666–82.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  38. Ettaki H, Troncoso-Ponce MA, To A, Barthole G, Lepiniec L, Bauda S. Overexpression of MYB115, AAD2, or AAD3 in Arabidopsis thaliana seeds yields contrasting omega-7 contents. PLoS One. 2018;13(1):e0192156.

    Article  PubMed  PubMed Central  Google Scholar 

  39. Cakir A. Essential oil and fatty acid composition of the fruits of Hippophae rhamnoides L. (sea buckthorn) and Myrtus communis L. from Turkey. Biochem Syst Ecol. 2004;32:809–16.

    Article  CAS  Google Scholar 

  40. Dulf FV. Fatty acids in berry lipids of six sea buckthorn (Hippophae rhamnoides L., subspecies carpatica) cultivars grown in Romania. Chem Cent J. 2012;6:106.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  41. Ranjitha A, Sarin-Kumar K, Venugopalan VV, Arumughan C, Sawhney RC, Singh V. Fatty acids, tocols, and carotenoids in pulp oil of three sea buckthorn species (Hippophae rhamnoides, H. salicifolia, and H. tibetana) grown in the Indian Himalayas. J Am Oil Chem Soc. 2006;83(4):359–64.

    Article  Google Scholar 

  42. Yang CY, Liu XM, Chen ZY. Determination of fatty acid profiles in fifteen kinds of edible vegetable oil by gas chromatography-mass spectrometry. Food Sci. 2013;34(6):211–4.

    CAS  Google Scholar 

  43. Carlsson AS, Yilmaz JL, Green AG, Stymne S, Horfvander P. Replacing fossil oil with fresh oil - with what and for what? Eur J Lipid Sci Technol. 2011;113(7):812–31.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  44. Li RJ, Gao X, Li LM, Liu XL, Wang ZY, Lv SY. De novo assembly and characterization of the fruit transcriptome of Idesia polycarpa reveals candidate genes for lipid biosynthesis. Front Plant Sci. 2016;7:801.

    PubMed  PubMed Central  Google Scholar 

  45. Xue JA, Mao X, Wu YM, Yang ZR, Jia XY, Zhang L, et al. Expression of yeast acyl-CoA-∆9 desaturase for fatty acids biosynthesis in tobacco. Chin J Biotech. 2013;29(5):630–45.

    CAS  Google Scholar 

  46. Zhi Y, Taylor MC, Campbell PM, Warden AC, Shrestha P, El Tahchy A, et al. Comparative lipidomics and proteomics of lipid droplets in the mesocarp and seed tissues of Chinese tallow (Triadica sebifera). Front Plant Sci. 2017;8:1339.

    Article  PubMed  PubMed Central  Google Scholar 

  47. Yang B, Kallio H. Analysis of triacylglycerols of seeds and berries of sea buckthorn (Hippophae rhamnoides) of different origins by mass spectrometry and tandem mass spectrometry. Lipids. 2006;41(4):381–92.

    Article  CAS  PubMed  Google Scholar 

  48. Ding J, Ruan CJ, Bao YH, Guan Y, Shan JY, Li H, et al. Analysis of genetic relationships in sea buckthorn (Hippophae rhamnoides) germplasm from China and other countries using ISSR markers. J Hortic Sci Biotechnol. 2015;90(6):599–606.

    Article  CAS  Google Scholar 

  49. Shanklin J, Somerville C. Stearoyl-acyl-carrier-protein desaturase from higher plants is structurally unrelated to the animal and fungal homologs. Proc Nat Acad Sci U S A. 1991;88:2510–4.

    Article  CAS  Google Scholar 

  50. Du HW, Huang M, Hu JY, Li JS. Modification of the fatty acid composition in Arabidopsis and maize seeds using a stearoyl-acyl carrier protein desaturase-1 (ZmSAD1) gene. BMC Plant Biol. 2016;16:137.

    Article  PubMed  PubMed Central  Google Scholar 

  51. Nguyen HT, Mishra G, Whittle E, Pidkowich MS, Bevan SA, Merlo AO, et al. Metabolic engineering of seeds can achieve levels of omega-7 fatty acids comparable with the highest levels found in natural plant sources. Plant Physiol. 2010;154(4):1897–904.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  52. Gao CY, Mao X, Shang HQ, Li F, Hao JY, Zhang L, et al. Effect of overexpression of MucACP-Δ9 desaturase on lipid synthesis in tobacco leaves tissuse. Plant Physiol J. 2016;52(9):1333–40.

    Google Scholar 

  53. Li SS, Wang LS, Shu QY, Wu J, Chen LG, Shao S, et al. Fatty acid composition of developing tree peony (Paeonia section Moutan DC.) seeds and transcriptome analysis during seed development. BMC Genomics. 2015;16:208.

    Article  PubMed  PubMed Central  Google Scholar 

  54. Pidkowich MS, Nguyen HT, Heilmann I, Ischebeck T, Shanklin J. Modulating seed beta-ketoacyl-acyl carrier protein synthase II level converts the composition of a temperate seed oil to that of a palm-like tropical oil. Proc Natl Acad Sci U S A. 2007;104(11):4742–7.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  55. Zhao N, Zhang Y, Li Q, Li R, Xia X, Qin X, et al. Identification and expression of a stearoyl-ACP desaturase gene responsible for oleic acid accumulation in Xanthoceras sorbifolia seeds which plays a key role in determining the ratio of saturated to unsaturated fatty acids. Plant Physiol Bioch. 2014;87:9–16.

    Article  Google Scholar 

  56. Sellwood C, Slabas AR, Rawsthorne S. Effects of manipulating expression of acetyl-CoA carboxylase I in Brassica napus L. embryos. Biochem Soc Trans. 2000;28(6):598–600.

    Article  CAS  PubMed  Google Scholar 

  57. Ruan CJ, Li Q. Status of oil content control in seed by genetic regulation and the strategy for improving the gene of Kosteletzkya for biodiesel production. Renew Energy Resour. 2008;26(4):35–40.

    CAS  Google Scholar 

  58. Remize F, Barnavon L, Dequin S. Glycerol export and glycerol-3-phosphate dehydrogenase, but not glycerol phosphatase, are rate limiting for glycerol production in Saccharomyces cerevisiae. Metab Eng. 2001;3:301–12.

    Article  CAS  PubMed  Google Scholar 

  59. Zhang L, Wu P, Lu W, Lü S. Molecular mechanism of the extended oil accumulation phase contributing to the high seed oil content for the genotype of tung tree (Vernicia fordii). BMC Plant Biol. 2018;18:248.

    Article  PubMed  PubMed Central  Google Scholar 

  60. Guo HH, Wang TT, Li QQ, Zhao N, Zhang Y, Liu D, et al. Two novel diacylglycerol acyltransferase genes from Xanthoceras sorbifolia are responsible for its seed oil content. Gene. 2013;527(1):266–74.

    Article  CAS  PubMed  Google Scholar 

  61. Vigeolas H, Waldeck P, Thorsten Z, Geigenberger P. Increasing seed oil content in oil-seed rape (Brassica napus L.) by over-expression of a yeast glycerol-3-phosphate dehydrogenase under the control of a seed-specific promoter. Plant Biotechnol J. 2007;5(3):431–41.

    Article  CAS  PubMed  Google Scholar 

  62. Zhao Y, Liu M, He L, Li X, Wang F, Yan B, et al. A cytosolic NAD+-dependent GPDH from maize (ZmGPDH1) is involved in conferring salt and osmotic stress tolerance. BMC Plant Biol. 2019;19:16.

    Article  PubMed  PubMed Central  Google Scholar 

  63. Chhikara S, Abdullah HM, Akbari P, Schnell D, Dhankher OP. Engineering Camelina sativa (L.) Crantz for enhanced oil and seed yields by combining diacylglycerol acyltransferase1 and glycerol-3-phosphate dehydrogenase expression. Plant Biotechnol J. 2018;16:1034–45.

    Article  CAS  PubMed  Google Scholar 

  64. Jako C, Kumar A, Wei Y, Zou J, Barton DL, Giblin EM, et al. Seed-specific over-expression of an Arabidopsis cDNA encoding a diacylglycerol acyltransferase enhances seed oil content and seed weight. Plant Physiol. 2001;126:861–74.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  65. Savadi S, Naresh V, Kumar V, Bhat SR. Seed-specific overexpression of Arabidopsis DGAT1 in Indian mustard (Brassica juncea) increases seed oil content and seed weight. Botany. 2015;94(3):177–84.

    Article  Google Scholar 

  66. Ding J, Wang L, Ruan CJ. Comparative transcriptome analysis of lipid biosynthesis in seeds and non-seed tissues of sea buckthorn. Genes Genom. 2017;39:1021–33.

    Article  CAS  Google Scholar 

  67. Vanhercke T, Tahchy AE, Shrestha P, Zhou XR, Singh SP, Petrie JR. Synergistic effect of WRI1 and DGAT1 coexpression on triacylglycerol biosynthesis in plants. FEBS Lett. 2013;587:364–9.

    Article  CAS  PubMed  Google Scholar 

  68. Shen B, Allen WB, Zheng P, Li C, Glassman K, Ranch J, et al. Expression of ZmLEC1 and ZmWRI1 increases seed oil production in maize. Plant Physiol. 2010;153:980–7.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  69. Liu J, Hua W, Zhan G, Wei F, Wang X, Liu G, et al. Increasing seed mass and oil content in transgenic Arabidopsis by the overexpression of wri1-like gene from Brassica napus. Plant Physiol Biochem. 2010;48(1):9–15.

    Article  CAS  PubMed  Google Scholar 

  70. Focks N, Benning C. Wrinkled1: a novel, low-seed-oil mutant of Arabidopsis with a deficiency in the seed-specific regulation of carbohydrate metabolism. Plant Physiol. 1998;118:91–101.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  71. Shan JY, Ding J, Wu YX. The evaluation for introduced sea buckthorn adaptation of the third Russia’s generation in northeast black soil area. Global Seabuckthorn Res Dev. 2014;12(4):5–9.

    Google Scholar 

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Acknowledgements

The authors thank Jinyou Shan and Ke Tang for their assistance in collecting plant materials.

Funding

This work was financially supported by the National Natural Science Foundation of China (31570681 and 31800574). CR, the funder of NSF (31570681) conceived this study. JD, the funder of NSF (31800574), Dalian Minzu University Doctor Scientific Research Foundation (0701110152), and University Innovation Groups Foundation of Dalian (201865) performed the experiments and wrote the manuscript.

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The datasets used and/or analyzed during the current study are available from the corresponding author on reasonable request.

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Contributions

JD designed and carried out the experiment of this study, and wrote the manuscript. CR conceived the study. WD and YG carried out the experiment of this study. All authors read and approved the manuscript in its final form.

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Correspondence to Chengjiang Ruan.

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Additional files

Additional file 1:

Table S1. Primers used for qRT-PCR of multigenes involved in FA biosynthesis and TAG accumulation in sea buckthorn berry pulp. (XLSX 14 kb)

Additional file 2:

Table S2. FA composition in sea buckthorn berry pulp from lines ‘Za56’ and ‘TF2–36’ at four developmental stages. (XLSX 13 kb)

Additional file 3:

Table S3. Functional classifications of unigenes in developing berry pulps of sea buckthorn based on COG category and KEGG pathway. (XLSX 12 kb)

Additional file 4:

Table S4. Differentially expressed genes for each of the 16 pairwise comparison groups. (XLSX 3263 kb)

Additional file 5:

Table S5. Unigenes involved in FA biosynthesis and TAG accumulation based on KEGG pathway analysis. (XLSX 44 kb)

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Ding, J., Ruan, C., Du, W. et al. RNA-seq data reveals a coordinated regulation mechanism of multigenes involved in the high accumulation of palmitoleic acid and oil in sea buckthorn berry pulp. BMC Plant Biol 19, 207 (2019). https://0-doi-org.brum.beds.ac.uk/10.1186/s12870-019-1815-x

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